The Determination And Significance Of The Polycyclic Aromatic Hydrocarbons In Human Lung Cancer

Objective:To established a method of extracting and detecting polycyclic aromatic hydrocarbons(PAHs) in human lung tissue and evaluate the association of PAHs and human lung cancer.This may contribute to explore the chemical and pathogenic factors of lung cancer,and to provide theoretical and experimental basis for further study lung cancer.Methods:15 human lung cancer tissues and 13 human normal lung tissues,all confirmed by pathology,were randomly recruited.The human lung tissues were extracted by ultrasonic wave and cleaned up by solid phase extraction.After concentrated and filtrated,the PAHs were analyzed by HPLC.Results:The calibration curves of the method were linear within the range of 1.2～392.2ng/ml,the detection limits ranged from 0.6 to 4.2ng,the recovery ranged from 68.6%to 103.7%with the RSD less than 10%.PAHs could be detected in all lung tissue.The differences of the content of each PAHs between lung cancer and adjacent tissue are not significant(q=0.5876～0.9873, p＞0.05).The contents of 2-methyl anthracene,pyrene,fluoranthene and benzo(a) pyrene in lung cancer tissue and adjacent tissue are higher than lung tissue without lung cancer, the differences are statistically significant(F=3.65～6.63,q=3.1911～4.8692,P＜0.05).Conclusion:(1)A method of extracting and detecting polycyclic aromatic hydrocarbons(PAHs) in human lung tissue was established.(2) It is the first time that PAHs were found in human lung tissues.(3)Human lung tissue has extremely strong ability of bioconcentrating PAHs.(4)There are high association of PAHs and occurrence of human lung cancer.PAHs may be one of the most important chemical factors to lead to human lung cancer.