| Objective:To investigate the protection of Rg2 on hypoxic cardiocytes.To study the pathogenesis of the Rg2 on myocardial cells against anoxia in vitro,in order to provide according to clinical medication.Methods:Cardiocytes of 24 hours old newborn rat were cultured for four days then divided into normal group,model group and Rg2 group.Rg2(0.025mmol/L) was added to Rg2 group,and culture fluid was added to the normal group and model group.All were incubated for four hours,and then model group and Rg2 group were taken to 37℃homeot hermic hermetic container,and given 95%N2 and 5%CO2 and mixed.The morphological changes were observed and Trypan Blue uptake rate of myocardial cells was calculated by Trypan Blue staining method.The apoptosis of cardiocytes were detected by flow cytometry(FCM).The contents of MDA and the activity of SOD,LDH and GXH-Px were determined by biochemical method.Results:①Compared with the normal group,Trypan Blue uptake rate of myocardial cells significantly increased in the model group(P＜0.05).Compared with the model group, Trypan Blue uptake rate of myocardial cells significantly decreased in the Rg2(P＜0.05).②The apoptosis significantly decreased in the Rg2 and the normal groups(P＜0.05).③Compared with the model group,the activity of SOD,LDH and GXH-Px and the contents of MDA in Rg2 group and normal group showed statistical significance(F=5.67-13.72, q=4.35-13.68,P＜0.05).Conclusion:The protective effect of Rg2 on myocardial cells against anoxia in vitro possibly through inhibiting cell apoptosis,increasing the energy of antioxidase and eliminating free radicles. |
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