Experimental Study On The Effect Of SJAMP On Apoptosis Of Human Hepatocellular Carcinoma Cell Line HepG2 In Vitro

Objective Through studying the apoptosis induced by SJAMP in the hepatocellular carcinoma cell line HepG2 in vitro,finding its mechanism,and analysising the expression of P53,Bcl-2 and nm-23 in HepG2,To provide the theory foundation and its feasibility that whether it can be used for the chemotherapy of hepatocellular carcinoma.Methods The cells of HepG2 were cultured in vitro and treated with SJAMP at different doses(0.25,0.5,1.0,2.0,4.0mg/ml).MTT was used to observe the inhibitory effects of SJAMP on cell growth,Western Blot was used to detect apoptosis,and detect the apoptosis related change of expression of protein P53,Bcl-2 and nm23-H1.Results(1)SJAMP produced an obvious time-and-dose-dependent inhibitory effect on the HepG2 cells.(2)MTT identified that SJAMP can induce the apoptosis of HepG2 cells.(3)Western blot showed that SJAMP can induce the apoptosis of HepG2 cells through changing the expression of the protein of Bcl-2 and nm23-H1(P<0.05), comparing with the.(4)but SJAMP counts for nothing through P53 protein(P>0.05).Conclusion(1)SJAMP produced obvious inhibitory effects on HepG2 cells and induce HepG2 apoptosis.(2)SJAMP can enduce the anti-tumor function in the methord of changing the expression of protein Bcl-2 and nm23-H1.(3)SJAMP has no influence on protein P53 in the HepG2 cell.