Anti-Tumor Mechanism Analysis Of Arsenic Trioxide On The Subcutaneously Transplanted Tumor Of Human Ovarian Carcinoma In Nude Mice

Objective:To explore the inhibitory effect of arsenic trioxide(As₂O₃) on the growth of SKOV3 cell subcutaneous implanted tumor and its mechanisms.Methods:Nude mice were subcutaneously implanted with 4×10⁶ SKOV3 cells,then treated with intraperitoneal injection of different concentration of As₂O₃ and CBP,and the weight of implanted tumors,the tumor inhibition rates was calculated to evaluate the anti-tumor effect.The gene expression of caspase-3 was detected by the real-time RT-PCR.Results:As₂O₃ can inhibit the growth of the transplanted tumor in a dose-dependent manner.1.0mg/kg As₂O₃ and CBP groups could obviously inhibit the growth of transplantable tumor with the tumor inhibitory rate of 50.0%and 76.9%respectively.In contrast to the group of saline,the defference is significant(P<0.05).The expression of caspase-3 was up-regulated obviously in the 1.0mg/kg As₂O₃group in contrast to the saline group with the statistic difference(P<0.05 ).As₂O₃ showed the less haematological toxicity than CBP,and without hepatic and nephritic toxicity.Conclusions:As₂O₃ can inhibit the growth of human ovarian carcinoma implanted tumor, and its mechanisms are related to regulating up the activity of Caspase-3 and including the cell apoptosis in vivo.