Anti-Tumor Effect And Mechanism Analysis Of Arsenic Trioxide On The Subcutaneously Transplantable Tumor Of Human Cervical Carcinoma In Nude Mice

Objective:To study the anti-tumor effect and mechanism of arsenic trioxide on different concentration on the subcutaneously transplantable tumor of human cervical hela cell in nude mice in vivo.Methods:Nude mice were implanted with 4×106 cervical hela cell,Then they were divided at random into five groups:different concentration As2O3, saline and DDP group, which received intraperitoneal injection for ten days. The tumor inhibition rate was calculated to evaluate the anti-tumor effect..Liver,kidney function and the blood were tested. The apoptosis rate was detected by the flow cytometer.The relative expression of caspase-3 were detected by the Reverse transcription-polymerase chain reaction. Immunohistochemistry SP method was adopted to detect the expression of p-ERK.Results:Arsenic trioxide can inhibie the growth of the transplanted tumor.1.0mg/kg As2O3 and DDP groups could obviously inhibit the growth of transplantable tumor with the tumor inhibitory rate of 46.07% and 70.34% respectively. In contrast to the saline, the difference was significant(P<0.05)。In thel.Omg/kg As2O3 compared with the saline the expression of caspase-3 was up-regulated, with statistically differences (P<0.05); the expression of p-ERK between As2O3 groups and saline was down-regulated; As2O3 group compared with the DDP, did not show obvious hematology,hepatic and nephritic toxicity.Conclusion:As2O3 can inhibit the growth of the human cervical subcutaneous transplantable tumor by regulating up the expression of caspase-3 and inducing the cell apoptosis and regulating down the expression of p-ERK.