The Mechanism And Significance Of Rat Coronary Vessel Microthrombosis In Situ

AIM: Making a model of rat coronary vessel microthrombosis in situ byaurostearic acid sodium to validate repeatability of model and evaluate theamount and component of thrombus; Detecting the expression offgL2(fibrinogen-like protein 2 prothrombinase /fibroleukin)and theassociation with microthrombosis to explore the mechanism and significanceof coronary vessel microthrombosis in situ.METHODS: the 78 Sprague-Dawley male rats were randomly assigned tothe model group(n=36), sham operation group(n=36) for different time(1h,1d,1w,2w,3w,4w)( n=6,respectively), and the normal control group(n=6).After closing asceding aorta, laurostearic acid sodium was injected intofrom aortic root in the model group, unclamping asceding aorta 10s later;physiologic saline was injected into sham operation group instead,microthromb were detected with HE staining and Modified Martius-Sarlet-Blue Stains(MMSB); von Willebrand factor(vWF) was measured with ELISA ; fgl2 mRNA and protein expression were detected with RT-PCR,immunohistochemistry staining, respectively. cardiac structure and function were measured by echocardiogram..RESULTS: A lot of microthromb were observed in rat coronary microvessel(P=0.000), reaching the peak level at 1d; vWF increased; (P <0.05); fgl2 was expressed in the wall of coronary microvessel, reaching the peak level about between 1d and 1w(P <0.05), cardiac function was decreased at 1w, the structure was enlarged at 4w(P <0.05) in the model group which is comparable to the sham operation group, but no significant changes were observed in the sham operation group and normal control group(P >0.05).CONCLUSION: The model of rat coronary vessel microthrombosis in situ by aurostearic...