The Serum Proteomic Study Of Bone Metastases Of Lung Carcinoma And Prostate Carcinoma

Objective Using 2-D differential gel electrophoresis (2-D DIGE) and MS to screen serum biomarker candidates in lung cancer with bone metastases.Methods serum samples from 12 male lung cancer patients with bone metastases,12 male lung cancer patients without bone metastases,12 female lung cancer patients with bone metastases and 12 female lung cancer patients without bone metastases were obtained. Serum samples of the same group were pooled and the most abundant protein albumin was depleted in order to enrich low-abundance proteins and proteins with low molecular weight. Concentrated and desalted samples were labeled with three different CyDyes including one internal standard, pooled from all the samples, and separated with 2-D DIGE in triplicate experiments. Biological variations of the protein expression level were analyzed with DeCyder software and evaluated for reproducibility and statistical significance. The differentially expressed protein spots in sera from lung cancer patients with bone metastases were analyzed by peptide-fingerprinting with matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS).Results A total of 11 protein spot-features were found to be significantly increased and 5 significantly decreased in lung cancer with bone metastases serum samples.The identified proteins included RBP-TTR complex that was decreased, haptoglobin, SNO-Hb, desArg141 alpha Hb that were increased in lung cancer with bone metastases serum samples.Conclusions The combination of 2-D DIGE and mass spectrometry (MS) strategy is an ideal platform and powerful approach for differential proteomic analysis of human sera. 4 proteins might be involved in the process of lung cancer bone metastases and improve our understanding of the fundamental chang that contribute to the process of carcinogenesis and the formation of metastases in lung cancer with bone metastases.. Objective Using the two-dimensional gel electrophoresis (2-DE) and mass spectromet ry to screen serum biomarker candidates in prostate cancer with bone metastases.Methods Serum samples from 5 prostate cancer patients with bone metastases,5 prostate cancer patients without bone metastases were obtained. The most abundant protein albumin in serum was depleted in order to enrich low-abundance proteins and proteins with low molecular weight. The samples were separated by two-dimensional electrophoresis (2-DE) and analyzed with ImageMaster 2D Platinum software.The differentially expressed protein spots in sera from prostate cancer patients with bone metastases were analyzed by peptide-fingerprinting with matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS).Results A total of 10 protein spot-features were found to be significantly increased and 5 significantly decreased in prostate cancer with bone metastases serum samples. The identified proteins included Zn-alpha2-glycoprotein(ZAG), haptoglobin and apolipop- roteinC-III that were increased in prostate cancer with bone metastases serum samples.Conclusion The combination of 2-D and mass spectrometry (MS) strategy is an ideal platform and powerful approach for differential proteomic analysis of human sera. 3 proteins might be involved in the process of prostate cancer bone metastases and improve our understanding of the fundamental chang that contribute to the process of carcinogenesis and the formation of metastases in prostate cancer with bone metastases.The proteins might be applied as biomarkers for bone metastases from prostate cancer.