The Study Of The Association Between PEMT Gene-774G/C Polymorphism And NAFLD

Aims To find out the correlation between PEMT gene -774G/C polymorphism and NAFLD in the Han nationality population in Fujian of China.Methods To collect the blood samples of nonalcoholic fatty liver disease patients and control subjects. To extract and to purify the genomic DNA from the collected blood sample.Using PCR-RFLP technique,the PEMT -774G/C polymorphism site was screened in 82 nonalcoholic fatty liver disease patients and 98 controls. To sequence the polymorphic gene found by RFLP technique. The representation of genotype of patients and controls was detected according to Hardy-Weinberg equilibrium. Genotypic,allelic frequencies and clinical index were compared between patients and controls,meanwhile clinical index was compared among different genotypes.Results For the PEMT polymorphism site of -774G/C,48 with the heterozygote mutation and 12 homozygote mutation were found in 82 NAFLD patients.The C allele frequency was 43.9% in NAFLD.41 with the heterozygote mutation and 8 homozygote mutation were found in 98 controls. The C allele frequency was 29.1% in control group.There was significant difference between two groups in the C allele frequency(P<0.01). In stratified analysis on the basis of sex,the frequencies of SNP PEMT–774 CC and GC genotype and C allele for NAFLD patients were significantly higher than those in controls(genotype:P < 0.05;allele:P < 0.01)in the female subjects.In both NAFLD group and control group,LDL-cholesterol,TG,FINS and HOMA-IR were significant higher in CC and GC genotype subjects than that in GG genotype subjects(P<0.05),HDL-cholesterol was significant lower in CC and GC genotype subjects than that in GG genotype subjects(P<0.01).Conclusions -774G/C variant of PEMT could be a candidate molecule that determines the susceptibility to NAFLD,and female subjects with -774G/C variant of PEMT were more facilitated to develop NAFLD. The C allele of PEMT gene -744 polymorphism site was regarded to be latently at risk of the metabolic syndrome.