Prediction Of Acute Heart Transplant Rejection In Rats With Protein Spectrum Technique

Objective: To use the protein spectrum technology, the adoption of heart transplantation in rats after acute rejection and transplantation proteomics study comparing serum proteomics research, to look for differences in protein.Methods: casing technology set up xenogeneic rat cervical heterotopic heart transplantation model. Acute rejection in groupâ… for receptor do not pre-processing, model set up five days after taking blood samples and the graft (donor heart) specimens; acute rejection groupâ…¡for receptor do not pre-processing, model building after 7 days take blood samples and the graft (donor heart) specimens; treatment group I, receptor 1 days preoperative intraperitoneal injection of cyclosporine A (CsA) 20mg/kg, every day after 10mg/kg, after five days of blood samples taken and the graft (donor heart) specimens; treatment group II, receptor 1 days preoperative intraperitoneal injection CsA20mg/kg, every day after 10mg/kg, after seven days from blood samples and graft (donor heart) specimens; blank group, check the health of Wistar rats before transplantation for blood and myocardial tissue specimens. Application of surface-enhanced laser desorption ionization time of flight mass spectrometry (surface enhanced laser desorption ionization time of flight mass spectrometry, SELDI-TOF-MS) technology to weak cation-chip (CM10) combination of serum protein and cardiac tissue proteins, for each group of Serum samples and myocardial tissue samples for protein mass spectrometry, Applied BioMarker Wizard and Biomarker Pattern software analysis in each group the differences between the protein and comparative analysis of differences in protein peak intensity.Results: The experimental group and blank group in serum and myocardial tissue extract using PBSâ…¡MS (CM10-chip) data collection, application Biomarker Wizard software for all serum and myocardial tissue proteins standardization, then analysis of the blank group and the serum protein peak number and relative intensity, blank group and acute rejection groupsâ… screened seven statistical differences in protein were the 2871 Da, 5613 Da, 6973 Da, 14893 Da, 3438 Da, 3965 Da, 4312Da. At the blank group and the acute rejection groupâ…¡selected 11 proteins with significantly different protein , 2596Da, 3438Da, 3965Da, 4312 Da, 5495Da, 5613Da, 6973Da, 8283Da, 14893Da, 15011Da, 15887Da. Acute rejectionâ… and treatment of groupâ… , four protein peaks with statistical significance, 3438 Da, 4312 Da, 5613 Da, 6973 Da. Acute rejectionâ…¡and treatment of groupâ…¡, six protein peaks with statistical significance, M/Z were 3438Da, 3965Da, 4312Da, 5613Da, 6973Da, 14893Da. Each experimental group and blank l group of cardiac transplantation in myocardial tissue protein extract samples mass spectrometry, in the blank group and acute rejection groupsâ… selected seven proteins with significantly different peaks, M/Z were 2009Da, 4787Da, 5176Da, 7610Da, 8134Da, 10821Da, 12364Da. At the blank group and the acute rejection groupâ…¡selected 13 proteins with significantly different peaks, M/Z were 3499Da, 4209Da, 4787Da, 5176Da, 7033Da, 7610Da, 8134Da, 8976Da, 9652Da, 10821 Da, 12364 Da, 13067Da, 15213Da. And treatment of acute rejectionâ… , four protein peaks with statistical significance, M/Z were 4787Da, 5176Da, 7610Da, 8134Da. And treatment of acute rejectionâ…¡, the 7 protein peaks with statistical significance, M/Z were 3499Da, 4787Da, 5176Da, 7610Da, 8134Da, 8976Da, 10821Da.Conclusions: By differences in serum protein expression profiling analysis, the M/Z were 3965Da, 3438 Da, 14893 Da protein may be associated with the early stages of acute rejection , the M/Z were 8283 Da, 15011 Da, 15887 Da protein is probably prediction of acute rejection may have entered the peak period. the M/Z were 4312 Da, 5613 Da, 6973 Da protein peak could be possible changes in prediction of the occurrence of acute rejection and development. Heart transplantation in myocardial tissue of the differences in protein expression profile analysis, the M/Z were 4787Da, 5176Da, 7610Da, 8134 Da, 10821Da possible changes in the protein peak can be predicted the occurrence of acute rejection and development. the M/Z was 12364Da protein may be associated with the early stages of acute rejection, the M/Z were 3499Da, 4209Da, 7033Da, 8976 Da, 9652 Da, 13067 Da, 15213 Da protein may predict acute rejection has entered the peak period . Proteomics technology in the prediction of acute heart transplant rejection may be an extensive clinical application.