| Objective:According to statistics,most patients with liver cirrhosis are associated with intestinal endotoxemia(IETM), a large number of endotoxin in vivo aggregation is an important reason for his condition to deteriorate.In the early prevention and treatment of acute hepatic encephalopathy experimental study,the respected Professor Zhang Chizhi Ru Huang preraration found significantly lower serum levels of endotoxin.Dr.Lu Dingbo and experimental research in the post-specific data through a more precise analysis of the conclusions as follows:normal group,prevention group,serum levels of governance mode endotoxin levels were significantly lower than the liver cirrhosis model group(P＜0.05),and normal group,prevention group,governance mode between the endotoxin level was no significant difference(P＞0.05),treatment group and control group over the same period between the treatment of endotoxin level had no significant difference(P＞0.05). Therefore,we will build on the experimental rat model of liver cirrhosis,from the molecular level control of the milk preparation Huang intestinal endotoxemia done to explore deeper.Therefore,we will build on the experimental rat model of liver cirrhosis,from the molecular level of Ru Huang preraration to combat intestinal endotoxemia further explored.Methods:1.SPF the level male Wistar big mouse(200-250 grams) 88,divide into 6 groups stochastically.Normal group(n=8), model group(n=24),prevention group(n=16),prevention control group(n=16),model treatment group(n=12),model treatment control group(n=12).2.Besides the normal group,other each group uses the CcL4 compound factor law manufacture liver cirrhosis model,raises 10 weeks.3.Makes at the same time the mold,the normal group,the model group give the physiological saline to fill the stomach according to the 1ml/100g proportion;The prevention group,the prevention control group is separately giving the breast Ru Huang preparation,the young fructose also presses above proportion to fill the stomach 10 weeks;Makes the mold 9th week to start separately to the model treatment group,the model treatment control group to give the breast Ru Huang preparation, the young fructose according to above proportion fills the stomach continual 2 weeks.4.10th weekend big mouse execution selection.(1) takes the liver left leaf to make the liver organization pathology examination;(2) determines in each group of big mouse blood serum through the NO examination reagent box the NO content; (3) Rat TNF-αELISA kit Quantitative determination of liver tissue tumor necrosis factor-content.Results:(1) Ru Huang preraration can improve the prevention and treatment group model of liver cirrhosis and liver tissue fibrous tissue hyperplasia,the degree of inflammatory cell infiltration,including prevention group the pathological changes in liver tissue to improve the most obvious.(2) Ru Huang preraration can be significantly reduced in rats with cirrhosis of the level of NO in serum(P＜0.05).(3) Ru Huang preraration can be reduced cirrhosis of rat liver tissue TNF-αexpression levels,thereby reducing liver injury,directly or indirectly.Conclusion:In the experimental study found that serum endotoxin levels and liver inflammation and liver damage there was a clear positive correlation.Ru Huang preraration on the one hand,the promotion of intestinal excretion of toxins, reducing the accumulation of toxins in the intestinal tract; on the other hand,acidification of the gut have added intestinal bacteria,gut microflora balance of the environment, improve the intestinal blood perfusion,and promote intestinal cell proliferation to reduce the intestinal mucosal villous epithelial shedding shorter,reducing the permeability of intestinal mucosa,reducing the absorption of endotoxin to enter the blood circulation of endotoxin decreased significantly,and the inflammatory response in liver tissue and liver damage also alleviate.
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