Effects Of Ruhuang Preparation On Intestinal Endotoxemia Of Liver Cirrhosis Rats

Objective Intestinal endotoxemia(IETM)in various liver diseases,particularly severe cirrhosis of the liver and the high incidence of hepatitis.Patients with cirrhosis due to intestinal flora imbalance,impaired barrier function,increased bacterial translocation,the doors of segregation,and liver cells,Kupffer cell dysfunction, endotoxin(LPS)degradation ability to gut the inside Toxins into the large number of peripheral circulation.IETM lead to liver damage mechanism is very complicated and can damage the liver cells,can also activate Kupffer cells,macrophages single-core to release pro-inflammatory media indirect damage liver cells,can induce systemic metabolism and blood flow dynamics disorder,caused systemic organ Failure,there ascites,hepatic encephalopathy,liver and kidney syndrome and other complications of the occurrence and development,which has seriously affected the prognosis of patients.Chinese medicine theory,not gut endotoxemia the devoted,but according to their pathogenic features of the "intrinsic evil drug" areas.Mentor Professor Zhang Chizhi According to Chinese medicine theory and clinical experience that the pathogenesis of this disease is due to liver spleen and kidney dysfunction,endogenous dampness and water retention,block blood stasis,wet stasis among guitar,pathogen accumulation causing fire into drugs.Commit evil of liver toxicity,liver losing regulating,spleen losing transportation and transformation,stolen intestinal conduction Division,Fu cloud of Health,the disease of the liver and intestine.This legislation "-the antidote laws" in light of "the same liver and large intestine,liver disease to clear the large intestine," the academic thinking,wine selection system composed of lactic acid bacteria and RuHuang preparation of cirrhosis IETM.We in the early prevention and treatment of acute hepatic encephalopathy in the experimental study found that RuHuang agents(the Hubei Provincial Health Department funding issues)can reduce the plasma levels of toxins,more experimental rat control group,model,lactulose significant role in the control group.To further study the drug in the prevention and treatment of liver cirrhosis IETM mechanism,we will discuss cellular and molecular level.Methods SPF level of 88 male Wister rats,weight 200 to 230 grams,were divided into six groups:the normal group,model group,prevention group,the prevention of the control group,model treatment group,the model of control Group.In addition to the normal group,other groups of factors are used CcL4 cirrhosis of manufacturing model,keeping 10 weeks.The normal group,the model group distilled water 2 ml/d gavage;prevention group,prevention model in the control group were also given RuHuang formulations,lactulose 2 ml/d gavage;model first nine weeks beginning of the treatment group model,the model for the control group were given RuHuang, lactulose 2 ml/d gavage for two weeks.Article 10 killed in weekend material.(1)in the absence of heat sources under the condition of the right ventricle-5 ml of blood testing blood levels of endotoxin(2)semi-quantitative reverse transcriptasepolymerase chain Act(RT-PCR)testing of liver tissue CD14mRNA,TLR-4mRNA, LBPmRNA The expression(3)of serum D-lactic acid,diamine oxidase(DAO)the level of understanding of the permeability of the intestine(4)from the small intestine extract of 200μl,RIA-automatic application of the sample group of enterovirus Mucosa secretory immunoglobulin A(S-IgA)concentration understanding of the intestinal barrier function of the immune(5)take 0.1 g of the right of liver,0.1 g of the spleen with saline wash after the bacterial culture to observe the intestinal bacteria Shift the situation.(6)take the left of liver disease to do the examination.Results(1)RuHuang agents can significantly reduce the level of toxins in the liver cirrhosis in rats(P＜0.05);(2)RuHuang preparations can be lowered rat liver cirrhosis LBPmRNA,CD14mRNA,TLR4mRNA the expression level,thereby reducing the toxin directly on the liver Or indirect harm,in particular model effect of the treatment group better;(3)RuHuang agents can significantly reduce cirrhosis of serum D-lactic acid,DAO level(P＜0.05),lower intestinal permeability,especially in the prevention of significant effects of group;(4)RuHuang preparation can effectively improve the intestinal mucosa S-IgA secretion levels(P＜0.05),good effects of early intervention; (5)prevention group after RuHuang agents did not appear after the intervention of bacterial translocation situation;(6)RuHuang preparation can Improve prevention group and model treatment group of liver cirrhosis and fibrous tissue proliferation, inflammatory cells invasion,prevention group the pathological changes in liver tissue to improve significantly.Conclusion RuHuang preparation of cirrhosis of the role of endotoxemia,there may be mechanisms to achieve the following:①remove intestinal toxins,and also by inhibiting LPS LPS-binding protein(LBP)and the corresponding receptor CD14, TLR-4 Combine to reduce the toxins in the intestinal absorption;②increase of intestinal immune function,effectively prevent the harmful bacteria to the intestinal mucosa of adhesion,the protection of the intestinal mucosa,to prevent bacterial translocation.③improve intestinal perfusion,and promote intestinal cell proliferation and reduce the intestinal mucosa villi epithelial shedding shorter,reducing the permeability of the intestinal mucosa;④added the profitable fungus of intestinal, curb the excessive growth of bacteria,balance the microbiology of the gut environment,Reduce endotoxemia.