The Experiment Research Of The Culcure Of Articular Chondrocytes From Rabbits And The Effect Of SNP On Apoptosis Of Chondrocytes

OBJECTIVE:To master the operation of related equipment,to train the experimental concept of asepsis,to carry out the separation and culture of articular chondrocytes from rabbit,to observe the biological patter of articular chondrocytes from rabbit in vitro, to carry out the experiment of of articular chondrocytes from rabbit induced by different concentrations of sodium nitroprusside(SNP) and different times,to calculate of the rate of apoptosis,to explore the mechanisms of apoptosis of articular chondrocytes induced by sodium nitroprusside(SNP) and the mechanisms of occurrence between apoptosis of articular cartilage and osteoarthritis(OA).METHODS:1.Operate the different devices in the laboratory..2.Carry out the training of aseptic operation in the laboratory.3.Make up a variety of experimental reagents in clean console.4.Carry out the separation and culture of articular chondrocytes from rabbit in CO₂ incubator.5.Carry out the culture of articular chondrocytes from rabbit in the CO₂ incubator. 6.Observe the biological morphology of articular chondrocytes from rabbit under the microscope.7.Carry out the experiment of of articular chondrocytes from rabbit induced by different concentrations of sodium nitroprusside(SNP) and different times.8.Detect the apoptosis of articular chondrocytes from rabbit by low cytometry.RESULTS:1.On biology of articular chondrocytes from rabbits with an initial understanding of the form.2.Set up the cartilage model of apoptosis successfully ihduced by NO donor SNP.3.Chondrocytes apoptosis and the SNP have a relationship between concentration and time.4.Use the inter-group design information combined analysis of variance is significant in statistics between the groups of different concentration and the groups of different times(p <0.01).5.Apoptosis of articular chondrocytes from rabbit induced by 2mmol /L concentration of sodium nitroprusside and cultured for 24 hours, the rate of apoptosis is significantly higher than other concentrations and other times of the control group(p <0.01).CONCLUSION:Establish the model of NO donor SNP-induced chondrocyte apoptosis sccessfully and found that SNP has a certain amount of efficiency and timeliness,to understand NO cause the apoptosis of articular chondrocytes and the mechanism of apoptosis of articular chondrocytes and OA.