Study On Pharmacognosy And Pharmacological Activity Of Bombyx Batryticatus

Quality evaluation of traditional Chinese medicine has been a difficult and multiple point on studying and applying for a long time,which has restricted the modernization and internationalization of traditional Chinese medicine.Bombyx Batryticatus is a traditional drug for calming the liver and subduing wind of Chinese medicine.Its original name is Jiang can.It can also be called Bai Jiangcan,Jiang Chong and Tian Chong.It is a dry and full body of 4-5 year old larva Bombyx mori L.which is origined from Bombycidae died of Beauveria bassiana (Bals.) Vaill.(or man-made).Bombyx Batryticatus is usually produced in spring,summer and autumn,which is mainly produced in Si Chuan province,Guang Xi province,Jiang Su province, Zhe Jiang province,An Hui province,Shan Dong province,Gan Su province and so on,and the quality of Si Chuan province is the best.Bombyx Batryticatus have effects such as dispelling wind,relieving convulsion,dissipate phlegm and eliminating stagnation.So,it is usually used for convulsion,Swelling of throat,skin titillation,lymphadenitis,facial paralysis and so on.It was described on characteristics,powder character,property and flavor meridian distribution, function and indication,usage and dosage in the Chinese Pharmacopeia of 2005.This paper had studied the pharmacognosy and pharmacological activity of Bombyx Batryticatus detailly, which increased new contents of Bombyx Batryticatus.On the pharmacognosy of Bombyx Batryticatus,we mainly studied its formation, collection and processing,characteristics,micro-character,physico-character and chemicharacters. This paper used Bombyx Batryticatus supplied by institute of silkworm in Si Chuan province as standard,and compared it with Bombyx Batryticatus bought from 10 different drugstores in Chang Chun,and discovered a new quality standard on identifying Bombyx Batryticatus by microsublimation.In this paper,according to the principle of microsublimation,we designed an new extracting device for sublimation.By the method,we could extract a great quantity of sublimation from Bombyx Batryticatus.The sublimation was identified as ammonium bicarbonate by the methods of high performance liquid chromatogram,infrared spectra and Xray diffraction.The acute toxicity test of the sublimation indicated that in the same dosage,the maximum tolerated dose of small mice was 4.0g/kg,when we used intragastric administration, and small mice twitched vigorously and died in a short time,when we used intraperitoneal injection.The median lethal dose was 0.812g/kg of intraperitoneal injection.The insecticidal activity test of the sublimation indicated that fumigating had an evident effect on Velar ifictorus rnicado(S.) and Tenebrio molitor(L.),The higher the does and temperature was,the better the insecticidal effect was.But in the condition of that insects could not accommodate,the control group had a different level of death.So megatemperature also had a large effect on Velarifictorus rnicado(S.) and Tenebrio molitor(L.).In the anti-convulsion test,compared with the control group,the water extract of Bombyx Batryticatus(high dose) could prolong the convulsion latency of small mice obviously(P<0.01); the sublimation of Bombyx Batryticatus(low dose) could prolong the death time of small mice obviously(P<0.01);but the water extract of Beauveria bassiana.(Bals.) Vuilllt(high dose) decurtated the convulsion latency and death time of small mice obviously.It showed the toxicity of Beauveria bassiana.(Bals.) Vuilllt,and that the sublimation was also an anti-convulsion component of Bombyx Batryticatus.The sublimation and petroleum ether fraction were examined to the anti-tumor activity of Bombyx Batryticatus.The result indicated that the sublimation of Bombyx Batryticatus had some inhibiting activity to tumor.The inhibition ratio of low dose was 43.56%and which of high dose was 36.59%.But we discovered that spleens of small mice became black.It showed that the sublimation had some negative effects on immune system of H₂₂ small mice.In the antibacterial activity test,the sublimation and petroleum ether fraction of Bombyx Batryticatus were examined.The result indicated that compared whith the control group,the sublimation had a very visible antibacterial activity to Escherichia coli ATCC25 923(P<0.01); The petroleum ether fraction had a very visible antibacterial activity(P<0.01) to Escherichia coli ATCC25 923,Escherichia coli E^R_ca1 and Staphylococcus aureus we selected In conclusion,the sublimation is a active substance of Bombyx Batryticatus.And there were also some active components which could inhibit Escherichia coli and Staphylococcus aureus in the petroleum ether fraction of Bombyx Batryticatus.We should separate them in the future.