Correlation And Mechanism Of CDK7 With The Development Of Endometrial Carcinoma

Objective:To investigate the expression and the clinical significance of CDK7 in endometrial carcinoma as well as their relationship with the clinicopathological factors.To investigate the relation of expression level between CDK7,cyclinH and P53 in endometrial carcinoma,so as to make a deeper conclusion about its function in the endometrial tumor's generation and development.Methods:Ninty-six patients with endometrial cancer,69 patients with normal endometrium,35 patients with endometrium intraepithelial neoplasia were studied their expression of CDK7 by immunohistochemistry.The expression level of CDK7, cyclinH and P53 was measured in 15 patients with normal endometrium,11 patients with endometrium intraepithelial neoplasia,23 patients with endometrial carcinoma by semi-quantity reverse transcription polymers chain reaction(RT-PCR),and the deeper analysis about the relation of expression depth on CDK7,cyclinH and P53 was made.Results:The positive expression rate of CDK7 in endometrial carcinoma was showed statistically higher than that in normal endometrium and in endometrium intraepithelial neoplasia(P＜0.05),it was proved having no statistically relationship with the pathological type and the histopathological grading of the endometial cancer in our artiale(P＞0.05),but having statistically positive relationship with the surgical pathologic staging,the depth of the cancer invading uterine myometrium,the lymph node metastasis and the positive ascites cytology(P＜0.05).The positive rate and average expression level of CDK7mRNA in the normal endometrium was 13.33%,0.3124±0.2834.The positive rate and average expression level of CDK7mRNA in the endometrium intraepithelial neoplasia was 18.19%,0.4220±0.2203.The positive rate and average expression level of CDK7mRNA in the endometrial carcinoma was 82.61%,0.5832±0.2343.The positive rate and average expression level of cyclinHmRNA in the normal endometrium was 20.00%,0.3840 0.1933.The positive rate and average expression level of cyclinHmRNA in the endometrium intraepithelial neoplasia was 27.28%,0.4178±0.2834.The positive rate and average expression level of cyclinHmRNA in the endometrial carcinoma was 78.26%,0.6241±0.3343.The positive rate and average expression level of P53mRNA in the normal endometrium was 33.33%,0.3840±0.2443.The positive rate and average expression level of P53mRNA in the endometrium intraepithelial neoplasia was 54.54%,0.4211±0.1946.The positive rate and average expression level of P53mRNA in the endometrial carcinoma was 86.96%,0.6412±0.3834.In the 23 patients with endometrial carcinoma,average expression of CDK7mRNA was positively correlated with cyclinHmRNA(r=0.319,P=0.008),and average expression of CDK7mRNA wasn't correlated with P53mRNA(r=0.130,P＞0.05).Conclusion:In our article,CDK7 was showed overexpression in endometrial carcinoma.The overexpression of CDK7 was correlated with the surgical pathologic staging,the depth of the cancer invading uterine myometrium,the lymph node metastasis and the positive ascites cytoloyg.The overexpression of CDK7 was proved being correlated with the progression and metastasis of the endometrial carcinoma. The positive rates and average expression levels of CDK7 and cyclinH in the endometrial carcinoma was significantly higher than that of in the normal endometrium and the endometrium intraepithelial neoplasia,and there was a positive correlation between CDK7 and cyclinH.It indicates CDK7 and cyclinH should participate the endometrial tumor's generation and development as a complex CDK7mRNA wasn't correlated with P53mRNA in the endometrial carcinoma.Our study preferd that CDK7 was useful tumor markers to evaluate the degree of malignancy and biological behavior for the endometrial carcinoma.