Preparation And Characteristics Research On The Affinity Matrices Fabricated By Conductive Polymers

This article fabricated two morphological affinity adsorb matrix by conductive polymers at first time:the poly(N-(2-carboxyethyl)pyrrole) microtubes and the poly(pyrrole-3-COOH)-AAO membrane,and researched on the morphous, physicochemical characteristic,adsorption,elution and regeneration.(1)Fabrication and characteristics research of the poly(N-(2-carboxyethyl) pyrrole) microtubes:The poly(N-(2-carboxyethyl)pyrrole) microtubes were fabricated by chemical oxidizing N-(2-carboxyethyl)pyrrole onto porous polycarbonate(PC) track-etch membrane using template method.Cu²⁺ was selected as ligand,chelating reagent IDA reacted with-COOH on the exterior and interior surface of the microtubes,the Cu²⁺ immobilized affinity microtubes with ideal structure was obtained.The Cu²⁺ content of affinity microtubes was 1.96 mg/g microtubes.Hemoglobin(Hb) was chosen as the adsorbate,the characteristics and adsorption mechanism of affinity microtubes were studied.The results were that:the prepared affinity microtubes showed good adsorption specificity for Hb;Higher adsorption capacity can be attained at lower ion intensity,higher initial concentration of Hb and neutral Hb solution(pH=6.8);the mechanisms of Hb adsorption on the affinity microtubes fitted the Freundlich model well,and R²= 0.999.The elution and regeneration of affinity microtubes were studied.The Hb adsorbed on the affinity microtubes can be totally eluted by the acetic acid-sodium acetate buffer(pH=3.6),and the elute rate was 99.63%.Cu²⁺ was eluted by EDTA, and the elute rate was 95%.After adsorption and elution experiment executed 10 cyclic,the adsorption capacity decreased 8%.(2)Fabrication and characteristics research of the poly(pyrrole-3-carboxylic) -AAO membrane:The poly(pyrrole-3-COOH)-AAO membrane was fabricated by vapor deposition polymerization(VDP) onto AAO membrane.Lysine was selected as ligand,through-NH₂ reacted with-COOH on surface of the membrane,the lysine content of affinity menmbrane was 5.86 mg/g membrane.Bilirubin(BR) was chosen as the adsorbate,the characteristics and adsorption mechanism of affinity membrane were studied.The results showed:the prepared affinity membrane showed good adsorption specificity for BR;Higher adsorption capacity can be attained at lower ion intensity,higher initial concentration of BR and lower albumin intensity;the mechanisms of BR adsorption on the affinity membrane fitted the Freundlich model well,and R²=0.99.The elution and regeneration of affinity membrane was studied.The BR adsorbed on the affinity membrane can be totally eluted by the NaOH and BSA,and the elute rate was 95%.The results showed:the different morphological affinity matrix fabricated by conductive polymers:the poly(N-(2-carboxyethyl)pyrrole) microtubes and the poly(pyrrole-3-COOH)-AAO membrane had favourable adsorption,desorption and regeneration function.