Experimental Study On The Effect Of Betaine On Ex Vivo Adipose Tissue Of Rats

Obesity is threatening the human health of chronic disease,increasingly becoming a serious harm to the global epidemic.With the socio-econimic prosperity,too much dietary caloric intake results in a degree of the conspicuous overweight and the thick fat layer,that is an obesity status caused by body adiposity particularly excessive accumulation of triglycerides. It is the most common serious impact on the human health of chronic non-communicable disease.Now,the main treatments of obesity were improvement of life style,exercise endurance,regular drug therapy and surgical treatment,etc.But these methods usually don't perform as good as at high bit.Therefore,to seek effective and safe drugs for weight loss and search for the weight reduction mechanism of medicine have become a hot subject.Betaine in the livestock feed attractant as agent of lean meat and add in the feed use. Betaine significantly inhibited calorie intake,food intake and body weight,decreased the serum MDA,leptin and IL-6,increased T-AOC of high fat fed rats in our pre-experimental study.But whether betaine has a direct impact on ex vivo adipose tissue would not have been reported at home and abroad.In the experiment,the ex vivo adipose tissues of rats were incubated under the given nutritional environments and specific circumstance,administered different drugs to observe the effect of free fatty acid(FFA) release,malondialdehyde(MDA), total antioxidant capacity(T-AOC),leptin,tumor necrosis factor-a(TNF-a) and interleukin-6 (IL-6) and preliminarily explored their possible functional mechanisms.Healthy adult SD rats weighing 250±20g were selected in the experiment. Experimental grouping as follows:normal control group,administered 0.9%physiological saline;adrenaline hydrochloride group,administered 10^-6 mol/L adrenaline hydrochloride; drug groups,including low-dose betaine(10^-7mol/L),middle-dose betaine(10^-6mol/L) and high-dose betaine(10^-5mol/L);mechanism group,middle-dose betaine separately administered with 10^-5mol/L propranolol,verapamil and SQ 22,536.Rats were executed by dislocation in the experiment.We used aseptic operation to take out the retroperitoneal adipose tissue, weighed,weight of each fat bolck about 50.0±2.0mg,and then cut into small pieces,which placed in 2ml Krebs solution with BSA(0.04g/ml) to incubate at 37℃,while respectively administered different concentrations of betaine and blockers plus betaine,including each drug capacity 20μl.Adipose tissue in the course of incubation were continuous access to 95% O₂ and 5%CO₂ mixed gas in order to make the PH value of solution remain at 7.4.After specimens incubating 90min,the supernatants samples were collected to measure the conteng of FFA,the level of malondialdehyde(MDA) and total antioxidant capacity(T-AOC) as well as the content of adipocytokines leptin,tumor necrosis factor-a(TNF-a) and interleukin-6(IL-6).SPSS 11.5 software was used to analyse the data and the results were expressed as Mean±SD((?)±S).The T-test was used for comparison between two groups.The One-Way analysis of variance(One-Way ANOYA) was used for comparison among the three groups. P＜0.05 represented significant differences.The main results were as follows:1 The effect of betaine and blockers plus betaine FFA release from ex vivo adipose tissue. Compared with normal control,adrenaline hydrochloride significantly increased FFA release from ex vivo adipose tissue of rats;low dose betaine had no significantly impact on the FFA release;however,middle dose betaine and high dose betaine could significantly promote the FFA release.Compared with middle dose betaine,that roles would partly be inhibited by propranolol blocking 6-adrenergic receptor,verapamil blocking L-type Ca²⁺ channel and adenylete cyclase inhibitor.2 The effect of betaine and blockers plus betaine on MDA and T-AOC from ex vivo adipose tissue.Compared with normal control,adrenaline hydrochloride significantly decreased MDA, but significantly increased T-AOC;similarly,MDA was decreased and T-AOC was increased in middle dose betaine.Compared with middle dose betaine,MDA was increased and T-AOC was decreased after administering propranolol,verapamil and SQ 22,536.3 The effect of betaine on leptin,interleukin-6(IL-6) and tumor necrosis factor-a(TNF-a) release from ex vivo adipose tissue. Compared with normal control,adrenaline hydrochloride had on significantly advanced role in leptin,IL-6 and TNF-a release from adipose tissue.However,middle dose betaine could obviously accelerate leptin release from adipose tissue,IL-6 and TNF-a had no significant differenceThe results indicate that betaine can significantly promote FFA release from ex vivo adipose tissue of rats,improve anti-oxidant capacity of body,increase leptin release.Their mechanisms of role could partly be through that the 6-adrenergic receptor on fat cells membrance promoted synthesis of cAMP from the upper reaches of PKA;or may be by virtue of opening Ca²⁺ channels on the cell membrance,causing the release of intracellular calcium and extracellular calcium influx,and further activated PKC,increased hormone-sensitive lipase(HSL) activity to achieve.