Comparision Of Hyperinsulinemic-euglycemic Clamp Adopting Different Methods In Rats

Objective To compare hyperinsulinemic-euglycemic clamp adopting venopuncture of double venae caudalis in rats without anesthesia with hyperinsulinemic-euglycemic clamp adopting other methods.To assess the stability and accuracy of the hyperinsulinemic-euglycemic clamp adopting venopuncture of double venae caudalis in rats without anesthesia.Methods 30 Wistar rats were randomly divided up into 2 groups and each group contained 15 rats.One group were fed with a standard chow diet and the other group were fed with a high-fat and high-sucrose diet (composition: 10% pig oil, 20% sucrose, 5% powder of yolk,65% normal feeds). They were fed in the SPF animal laboratory of Chinese traditional medical college of Gansu province. Eight weeks later,each group was randomly divided up into 3 small groups,each small group contained 4 rats and the spare 3 rats were taken as the substitutes.To perform 4 hyperinsulinemic-euglycemic clamps on each small group respectively.To compare the stability and the accuracy of these 4 methods.Results After venopuncture of vena caudalis, the blood glucose of the rats was in the normal range without any significant fluctuation, and no rat died; hyperinsuline -mic-euglycemic clamp adopting venopuncture of double venae caudalis in rats had a shortest period ,could be easiest done and had a highest successful rate.On the contrary, the catheterization of arteria carotis and vena cervicalis in rats had a highest death rate and longest period.The mean CV of GIR of hyperinsulinemic-euglycemic clamp adopting catheterization of arteria carotis and vena cervicalis in rats under anesthesia was significantly higher than that of other 3 methods. Performed the hyperinsulinemic-euglycemic clamp adopting catheterization of arteria carotis and vena cervicalis in rats under anesthesia, rats had a lower GIR than that of performed by other 3 methods. There was significant difference between hyperinsulinemic-euglycemic clamp adopting catheterization of arteria carotis and vena cervicalis in rats under anesthesia with other 3 methods(P<0.05).There was no significant difference among these 3 methods. GIR of the HD group was significantly lower than that of the ND group (P<0.01). BIns of HD group was significantly higher than that of ND group(P<0.01).Conclusion Comparing with these 3 techniques, the venopuncture of double venae caudalis technique can make hyperinsulinemic-euglycemic clamp in conscious rats be easier to perform, and have a shortened experimental period and low death rate of rats; the hyperinsulinemic-euglycemic clamp adopting catheterization of arteria carotis and vena cervicalis technique in rats under anesthesia was not stable.