The Change Of Cell Cycle And Proliferation After And Before On Locally Advanced Cervical Cancer Of Neoadjuvant Chemotherapy And Studying The Value Of Prediction Chemotherapeutical Sensitivity

Objective: To investigate the effect on in locally advanced cervical cancer on cell cycle, proliferation and apoptosis and proportion of cell in different phase, and to evaluate the feasibility of the therapeutical effect on neoadjuvant chemotherapy with proliferating cell nuclear antigen ( PCNA) on sensitive target.Methods: Retrospective analysis was done on the clinical data of 49 cases of neoadjuvant chemotherapy in locally advanced cervical cancer. Specimens were collected from pre-neoadjuvant chemotherapy biopsies and operative tissues. Flow cytometry (FCM) using PI staining was used to analyse the cell cycle , and the expression of proliferating cell nuclear antigen ( PCNA) also were examined by immunohistochemical staining.Results: The clinical therapeutic effect was evaluated after two weeks were treated in neoadjuvant chemotherapy for 49 cases of locally advanced cervical cancer. The clinical effective rate (CR+PR) was 79.6% (39/49), the operation rate in the post-neoadjuvant chemotherapy was 85.7%(42/49). The G0/G1 phase was primary possessed (62.71%±11.31%) in the pre-neoadjuvant chemotherapy, even to 83.5% , however the G2/M phase and apoptosis rate became increased, and the G0/G1 phase was decreased in the post-neoadjuvant chemotherapy. There was a significant statisticdifferences between the pre-neoadjuvant and post-neoadjuvant chemotherapy groups ( P<0.05). The S phase proportion of response group in the neoadjuvant chemotherapy was higher than that of no- response group, and there was a significant statistic difference between two groups , the former 21.47±5.21 and the latter 9.63±2.58 ( P < 0. 05) . The proliferation index of PCNA of response group in the neoadjuvant group in cervix pre- chemotherapy was higher than that of no- response group. Conclusion: The neoadjuvant chemotherapy was an important measure of locally advanced cervical cancer. The neoadjuvant chemotherapy may be effective to shrink the tumor size, relieve parametrial invasion, decrease staging of tumor, provid operation-chance and improve exairesis rate. The Neoadjuvant chemotherapy based on Cisplatin can inhibit proliferation and induce apoptosis and produce response of tumour cell through adjusting cell cycle kinetics of locally advanced cervical cancer. The S phase proportion and proliferation index of PCNA in pre-neoadjuvant chemotherapy were important indexes to assess chemotherapeutical sensitivity and therapeutical effect.