The Study And Application Of Energy Transfer Fluorescence Quenching In Inorganic Pollutants Determination

Objective:Fluorescence resonance energy transfer (FRET) is developed as a new method for determination of trace substances based on that electronic excitation energy transmitting between the suitable energy donor and energy receptor, which has been widly applied to the chemical analysis,the biological analysis,the biological structure studying and so on. In this paper, novel methods have been developed to determine the trace cadmium, nickel, copper, nitrite based on the fluorescence transformming intensity between the donor and receptor.Methods:In this work,PAN reacted with Cd2+, Ni2+ to form complexs which exist as energy receptors, and Rh6G existed as energy donor, novel fluorescence quenching assays were developed for the determination of trace Cd2+, Ni2+; so was the method for the determination of trace Copper that acridine red was used as donor and PAN-Cu2+ was used as receptor; another method was established for the determination of NO2- by the energy transfer system of AO- Rh6G,in which NO2- made the fluorence of Rh6G quenched.Results:In the system of PAN-Cd2--Rh6G,the detection limit for Cd2- was 9.0 ng/mL,the linear range was 31~800 ng/mL,and the r=0.9999. In the system of PAN-Ni2+-Rh6G,the detection limit for Ni2+ was 7.3ng/ml,the linear range was 24.3~640ng/ml,and the r=0.9996. In the system of PAN-Cu2+-AO,the detection limit for Cu2+ was 7.15ng/ml,the linear range was 23.8~600ng/ml,and the r=0.9996. And in the system of AO-Rh6G-NO2-,the detection limit for NO2- was 1.03ng/mL,the linear range was 3.39~1000ng/ml with r=0.9996. Conclusions:In this work new methods for determination of four kinds of inorganic ions have been established based on the energy transfer fluorescence quenching.The methods have the advartages of high sensitively, quickness and simplicity. They have been successfully emplyed to determine trace cadmium, nickel, copper and nitrite.