Study On The Spectrometry Of Resonance Rayleigh Scattering And Fluorescence For The Determination Of Meropenem And Hesperidin

Fluorescence analysis, as a routine analytical method, plays important roles in the field of trace elements, food testing, medicine, environmental protection and oil industry etc. It has some excellent properties of simplicity, convience, selectivity sensitivity, etc. The later developed fluorescence resonance energy transfer spec- troscopy provides a rapid simple and sensitive way to determine the structure of macromolecular substances and monitor the dynamic process. In recent years, fluorescence resonance energy transfer spectroscopy has solved some important issues emerging from the analysis and displayed good prospects, and adopted to the environmental and medical analysis.Resonance rayleigh scattering is a measurement of the fluorescence spectropho- tometer on the light scattering analysis. Synchronous rayleigh scattering spectrum can be gotten by selecting appropriate excitation and emission slit width on the fluorescence spectrophotometer, and using equal excitation and emission waveleng- ths to scan simultaneously, that's the so-called resonance rayleigh scattering.The studies are as follows:One is that meropenem and bromocresol green formed ion association complex in acidic medium by electrostatic interactions, which caused the absorbance of bromo- cresol green was decreased , but the resonance rayleigh scattering(RRS) intensity was enhanced significantly. The RRS intensity was directly proportional to the conce- ntration of meropenem in the range of 0.01¹4μg/mL with a detection limit of 8.08×10₃μg/mL. The method has been applied to the determination of meropenemin in plasma with satisfactory results.The second is that a new fluorescence quenching method for the determination of meropenem(MEPM)was developed, which was based on the effective energy transfer between fluorescein and eosin-Y(EY). In the presence of anionic surfactant cetyltrimethyl ammonium bromide(CTAB), the fluorescence intensity of EY was enhanc- ed by MEPM. Theâ–³F of system was directly proportional to the concentration of MEPM in the range of 0.5¹0μg/mL with a detection limit of 0.13μg/mL. The method has been applied to the determination of Meropenemin in urine with satisfactory results.The third is that a new method for the determination of hesperidin was developed. It is based on the enhancement of the intensity of fluorescence resulted from the Ion-association complex formed between hesperidin and aluminum (â…¢) in acid buffer medium, and the fluorescence intensity was enhanced significantly byβ-cyclodextrin. The change value of fluorescence intensity was directly proportional to the concentration of hesperidin in the range of 2.0×10₆ 4.0×10₄ mol/L with a detection limit of 2.6×10₇ mol/L. The method has been applied to the determination of hesperidin in muxiang shunqi pills with satisfactory results.The forth is that a new fluorescence quenching method for the determination of hesperidin was developed, which was based on the effective energy transfer between 1–naphthol(1-NP) and riboflavin (RF) . In the presence of cationic surfactant sodium dodecyl sulfate(SDS), the fluorescence intensity of RF was enhanced by Hesperidin. Theâ–³F of system was directly proportional to the concentration of hesperidin in the range of 0.1³0μg/mL with a detection limit of 9.7×10₂μg/mL. The method has been applied to the determination of hesperidin in tangerine peel with satisfactory results.