Effects Of Exercises On Immune Functions Of Murine Models With Chronic Fatigue Syndrome

Objective: To provide the experimental basis for studying the clinical exercise prescription of patients with chronic fatigue syndrome(CFS),the murine models built were used to investigated the effects of amount of exercise on the immune functions of murine models with CFS.Methods: Forty KM male mice were randomly divided four groups. They were comparative normal group(GroupⅠ, 10 mice), comparative CFS group(GroupⅡ,10 mice), exercise 1 group(GroupⅢ,10 mice) or exercise 2 group(GroupⅣ,10 mice). GroupⅡ,GroupⅢor GroupⅣwere forced to have 6-minute exhaustive swimming at the same time every day for three weeks. Then, GroupⅢor GroupⅣhad once or twice exercise for recover every day for two weeks. Weight, exhausted time or immovable time in suspension of every group were compared. After the murine eyeballs were picked, sera were abstracted and reserved in the refrigerator of -70℃. After execution by cervical vertebra dislocation, murine spleens taken with sterile operation were weighted. The spleen index were compared. The NK cells activities and lymphocytes stimulation index(SI) were measured by methyl thiazolyl tetrazolium (MTT) assay .The lymphocyte transformation rates were calculated by morphology detection. The circulating immune complexes (CIC)in sera were detected with PEG semiquantitative method. The content of inducible splenic IL-2 or IFN-γwas determined by ELISA, respectively. The splenic TNF-αwas detected by RT-PCR. The data were analyzed by statistics software SPSS13.0. When P<0.05, the difference was statistical significance.Results:(1) Murine weight Compared the weight of each group before the building of model, after the model built or post-treatment , the differences were non-significance (P>0.05).(2) Murine ethology index Calculated the immovable time in suspension or exhausted time of each group, the exhausted time or immovable time in suspension of GroupⅡ, GroupⅢor GroupⅣwere obviously longer than that of GroupⅠ( P<0.05). The exhausted time of GroupⅢor GroupⅣwas obviously shorter than that of GroupⅡ( P<0.05). The immovable time in suspension of GroupⅣwas obviously shorter than that of GroupⅡ( P<0.05).(3) Murine spleen index The spleen indexs of GroupⅡ, GroupⅢor GroupⅣwere obviously higher than that of GroupⅠ(P<0.05). The spleen indexs of GroupⅢor GroupⅣwas obviously lower than that of GroupⅡ( P<0.05).(4)Murine CIC in sera The contents of CIC in sera of GroupⅡor GroupⅢwas obviously higher than that of GroupⅠ(P<0.05). Compared GroupⅢwith GroupⅡ, the differences were non-significance(P>0.05). The contents of CIC in sera of GroupⅣwere obviously fewer than those of GroupⅡ(P<0.05).(5)The NK cells activities of mice The NK cells activities of GroupⅡor GroupⅢwere obviously lower than those of GroupⅠ(P<0.05). The NK cell activities of GroupⅢraised certainly, but were non-significance differences by compared with GroupⅡ(P>0.05). The NK cell activities of GroupⅣwere obviously higher than those of GroupⅡ(P<0.05).(6) Lymphocyte proliferation of mice The SI of murine lymphocyte of GroupⅡwas obviously lower than that of GroupⅠ(P<0.05). Compared the lymphocyte SI of GroupⅢor GroupⅣwith GroupⅡ, the differences were statistical significance(P<0.05). The lymphocyte transformation rates of GroupⅡor GroupⅢwere obviously lower than those of GroupⅠ(P<0.05). Compared the lymphocyte transformation rates of GroupⅢor GroupⅣwith GroupⅡ, those of GroupⅣwere obviously higher than those of GroupⅡ(P<0.05), but the difference were non-significance between GroupⅡand GroupⅢ(P>0.05).(7)Expression level of murine splenic IL-2 and IFN-γThe contents of splenic IL-2 or IFN-γof GroupⅡwere obviously lower than those of GroupⅠ(P<0.05). The differences were non-significance by compared the contents of IL-2 of GroupⅢwith GroupⅡ(P>0.05). The contents of IFN-γof GroupⅢwas obviously higher than that of GroupⅡ(P<0.05). The contents of murine splenic IL-2 or IFN-γof Group Ⅳwere obviously higher than those of GroupⅡ(P<0.05).(8) TNF-αdetection of mice Compared the contents of TNF-αof GroupⅡ, GroupⅢor GroupⅣwith GroupⅠ, the strap brightness of GroupⅡor GroupⅢwere obviously brighter than that of GroupⅠ. Compared GroupⅢor GroupⅣwith GroupⅡ, the strap brightness of GroupⅣwas obviously more dimmed than that of GroupⅡ. The strap brightness of GroupⅢwas nearly the same as that of GroupⅡ.Conclusions:(1) Murine models with CFS were successfully built.(2) Appropriate exercise might improve murine ethology index and spleen index, lower the contents murine CIC in sera, and increase the NK cells activities , lymphocyte SI or transformation rates. Meanwhile, it might adjust murine splenic IL-2 , IFN-γ, TNF-α.(3) Appropriate and regular exercise could be benefit for the recovery of mice with CFS.(3) The experimental results might provide the experimental basis for studying the clinical exercise prescription of the patients with CFS.