| Objective: To determine the distribution of Hepatitis B virus (HBV) genotypes and serotypes in community-based Zhengding area, Hebei province and evaluate the relationship between HBV genotypes and serotypes; analyze the spectra of mutations of the a determinant of HBV, study the relationship between the mutation and the vaccination; analyze the mutations of A1762T/G1764A and G1896A in the population. To describe the households clustering of HBsAg in Zhengding area, Hebei province by HBV epidemiology and molecular evidence.Methods: 11478 people's sera were collected by family survey and tested by SPRIA with kits in 2005 from Zhengding area, Hebei province, people's sera with positive HBsAg were collected and the S gene and C gene were amplified by PCR. After sequencing and clastwaling with the standard genotype sequences, the HBV genotype, serotype, the mutant sites of HBVαdeterminant and the A1762T/G1764A and G1896A mutations were detemined. 10011 people's sera from 3619 familys were collected and tested by SPRIA with kits. Negative-binomial distribution test were employed to fit distribution of the HBsAg. The Neighbor-Joining method was employed and the p-distance modle of MEGA 4.0 soft was selected to identify HBV transmission pairs with HBV S gene and C gene data. Statistical analyses were performed using Fisher exact test and X~2 test with SAS 8.1 software, P<0.05 was considered significant.Result: The study acquired 434 HBV S gene sequences and 393 HBV C gene sequences from 549 HBsAg positive samples eventually. The result of the study demonstrated that the B,C,D genotype have been detected in china and C is the major genotypes. Genotype C was 417 with serotype adr (95.2%), adw (3.6%), ayr (1.0%) and ayw (0.2%) ; Genotype B was 11 with adw (90.9%) and ayw (9.1%) and Genotype D was 6 with serotype ayw (66.7%) and adr (33.3%) . The mutation rate of the HBV a dominant was 6.7% in the community-based population and 9.4% in the population who born after the HBV immunization programe was carried out in this area. The 126,143 amino acid mutant rate is significant different between the two age groups. The BCP A1762T/G1764A and G1896A mutation rates are 40.5%, 18.1%. There is relationship between the HBeAg and the A1762T/G1764A mutation (P=0.0204) and the G1896A mutation (P=0.0003). 10011 sera samples from 3619 familys with definite household register were collected, the households clustering of HBsAg in Zhengding area is not fit the Negative-binomial distribution (X~2=6.75,P<0.05) . The clusting index is 0.64; Various kinds of HBV intrafamily transmission are indentified by the epidemiology and molecular evidence.Conclusion: The result of the study demonstrate that the B,C,D genotype and ayr,ayw,adr and adw serotype of HBV have been detected in Zhengding area,Hebei province. The C is the major genotypes and the adr is the major serotype. Genotype C with adr serotype, genotype B with adw and genotype D with ayw have the relationship. There are kinds of HBVαdeterminant mutations and 126,134 amino acid mutations are vaccine-associated mutation.A1762T/G1764A two-site mutation and G1896A mutation are the mostly common mutations in the HBV C gene region. There are virous kinds of intra-family transmission pattern in the area but the households cluster of positive serum of HBsAg does not exist. |
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