| Objective: To study the characteristics of distribution of genetic polymorphism in 15 specific STR loci (CSF1PO,D7S820,D8S1179,D21S11,D2S1338,D3S1358,D13S317,D16S539,TH01,D18S51,D19S433,TPOX,VWA,D5S818,FGA) and HLA (HLA-A, B and DRB1) alleles among healthy people at the age of life fragility.Methods: Healthy people aged between 50 to 70-year old were chosen as observed group. Their regular biochemical examination and blood examination was performed and the results were in the normal range. Each section of age has at least 1 case of male or female, but its maximum number is less than four. The total number is 93. Intravenous blood sample was collected (with one tube for EDTA anticoagulation and one tube for separation gel coagulation). DNA was extracted. The typing test for HLA-A, B and DRB1 (from 93 cases in the observed group) was performed by means of PCR-SSO and FCM. Fifteen specific STR loci (from 50 cases including 24 males and 26 females in the observed group) were tested by means of capillary electrophoresis. It was verified by determination of three special biochemical indicators that the choice of healthy people in the observed group is reasonable. The following published materials were used as control for HLA: the genotyping result of loci HLA-A, B and DRB1 from 9,678 volunteer stem cell donators aged between 18 to 45 in Dalian, who were healthy random suppliers with the nationality of Chinese Han (the typing method in this experiment is same to the published papers). One hundred and fifty healthy and non-related individuals (consisting of 75 males and 75 females) aged between 18 to 50-year old in Dalian were chosen as control for STR in the period of 2006 to 2008. Characteristics of the frequency distribution of both genetic locus groups and the homozygote frequency of each locus were compared and analyzed.Results: Fourty five specific genetic loci for HLA had been detected in the observed group, which include 10 HLA-A loci, 22 HLA-B loci and 13 HLA-DRB1 loci. The frequency of A*31 in the observed group was obviously higher than that in the control group (P<0.05). The frequency of A*11 was obviously lower than that in the control group (P<0.05). There is no significant difference between the frequency of HLA-B and HLA-DRB1 genetic loci in the observed group and the control group (P>0.05). The result of test for 15 specific STRs showed that the frequency of D3S1358-18, D13S317-11, VWA-20 and FGA-22 in the observed group was obviously higher than that in the control group and there was significant difference between the two groups (P>0.05), while the frequency of D18S51-14 and FGA-20 was obviously lower than that in the control group and there was also significant difference between these two groups. It was found out by homozygote frequency analysis of loci HLA-A, B and DRB1 and 15 STR loci that the actual frequency of each repeat homozygote of the locus D18S51 in the observed group was obviously lower than the theoretical frequency (P<0.05).Conclusion: A*31 in HLA may be a disease-resistant gene that contributes to the health. While A*11 may be a disease-susceptible gene. The genetic frequency of loci HLA-B and HLA-DRB1 dose not have closely correlation with the disease. There may be disease-resistant genes near four loci D3S1358-18, D13S317-11, VWA-20 and FGA-22 among 15 STR loci, while there may be disease-susceptible genes near the loci D18S51-14 and FGA-20. The heterozygote at the locus D18S51 may contribute to disease resistance.
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