A Elementary Study Of Meniscal Tears Repaired With Platelet-rich Plasma Autotransplantation Intraarticular Of Rabbit Knee

Objective: The purpose of this study was to observe whether rabbit autologous platelet-rich plasma (PRP) could promote rabbit healing of meniscal injure, explore its ability to promote healing of meniscal injure, and provide more information about PRP applied to repair meniscus injure and cartilage damage.Methods: In this study we chosed 20 healthy six-months male Chinese rabbits, two rabbits were used to verify the effectiveness of the minimally invasive meniscus injure model, the rest was randomly divided into three groups: group A,group B and group C, each group had 6 rabbits. And the lateral meniscus of each rabbit knees was used the minimally invasive approach to establish a damage model of transversal meniscus body, then use MRI (Atroscan ) to confirm the validity of meniscus damage. The left knee of each rabbit was defined the test side,While the right knee of each rabbit was defined the control side. After the left knee was injured, collecting autologous whole blood 7ml of each rabbit, then preparing about 1ml PRP by two centrifugation in constant temperature. Blood platelet count (BPC) was used to verify the degree of enrichment of PRP. After being suspended, then it was directly injected into the left knee articular cavity of each rabbit. All of the rabbit knee were given external fixation after surgery. Each rabbit was administered gentamicin to prevent infection for five days. The group A was sacrificed after being observed for one week, the group B was sacrificed after being observed for two weeks and the group C was sacrificed after being observed for three weeks. The lateral meniscus of each rabbit was evaluated by macropathology and histology, and then the result was statistically analyzed.Results: The meniscus injury of the experimental group A and the control group A didnt heal at one week after surgery. The meniscus injury of the experimental group B and the control group B didnt heal at two weeks after surgery. The meniscus injury of the experimental group C healed by granulation tissue and chondrocyte at three weeks after surgery; The meniscus injury of the control group C didNt heal at three weeks after surgery. The healing statistical analysis show that: with statistical significance (P <0.05).Conclusion: The rabbit autologous PRP applied to repair the avascular area injury of meniscus can stimulate cartilage cell proliferation at the edge of meniscus injury, and enhances the healing of the avascular area meniscal injury.