The Study Of Interaction Between Drug And Biomacromolecule Using Ion Probe And Co-Lumtechnology

The rapid development of analytical chemistry makes it break through of a single subject area, and accomplish the transition from analytical chemistry to the analytical science covering many subjects such as chemistry and mathematics, physics, computer science, biology, and developing as a multidisciplinary subject. One of the directions of development of analytical chemistry is to improve the sensitivity and selectivity of analysis, the anothers is to expand vigorously its space of development to life sciences field.Pharmaceutical analysis and biomacromolecule analysis have great development because of the superior of ion probe and co-lumtechnology. In this paper, a new determination method for drugs and serum albumin is established by utilizing ion fluorescence probe and co-lumtechnology, and study the interaction between drugs and biomacromolecule using photometry. The studies were performed as such follow: Part 1 The application of metalic ion probe technology on biological macro-molecule.(1) To study the spectrum behavior on the interaction of metalic ion-dye compositio and protein by metalic ion-dye probe method. The novel method for the determination of protein separately using o-Nitrophenylfluorone-Mo(VI) complex and arsenazo III-Tellurium(III) as spectroscopic probe was developed. Study the optimum conditions of systems, the coordinating groups of metallic ion and protein, and the microenvironment, spectrum behavior and mechanism of coordinating groups have been discussed in detail. Proposed analytic methods are simplicity convenience, high sensitinity and good selectivity,and the methods also can avoid background obstruction effectively,at the meantime,which were applied satisfactority to the determination of practical samples and the results are satisfied.(2) Based on the fluorescence enhancement of fluoroquinolone (pefloxacin,lavo-ofloxacin)-rare earths(Tb³⁺,Eu³⁺) complex by biological macro-molecule(seralbumin, ctDNA) , a sensitive fluorescence method for the determination of biological macro-molecule by fluoroquinolone-rare earths complex as spectroscopic probe were developed. Simultaneously, fluorescence enhancement mechanism of system and the interaction of between drugs and biomacromolecule have been discussed in detail. The probe is high sensitinity and good selectivity and environment friendly. These probes were applied satisfactorily to the determination of biological macro-moleculein (seralbumin, ctDNA) samples and the results were satisfied.Part 2 The application of the co-luminescence effect of rare earth in drug anaylsisCompared with single rare earth ion method, the rare earth co-luminescence method is of high sensitivity, good selectivity, information-rich and low determination limit, so it will win more and more attention. Currently,rare earth co-luminescence method was centralized used to analysis and determine rare earth,organic compound and luminescent material, however, which was not reported for pharmaceutical analysis. In this paper,we are using co-luminescence rare earth(Y³⁺-Eu³⁺) to determine drugs(sparfloxacin, metacycline) for the first time. The optimum condition of systems is attained and the interaction mechanism of co-luminescence systems was discussed by fluorescence spectra. The method was applied satisfactority on pharmaceuticals and the determination of biological samples and the results are satisfied.