| Objective In this study,the method for determining the in vitro content of ibuprofen was established,and two fluorescent probes were constructed as detection means,one is a D-?-A type fluorescent probe,and the other is a fluorescent probe which introducing a rare earth ion.The experimental conditions was optimized and a methodological study of the fluorescent probe method was carried out to create a convenient,accurate and efficient new method for determining the content of ibuprofen in vitro.Methods Firstly,a D-?-A type organic complex containing triphenylamine and three ternary rare earth complexes containing rare earth ions(Tb3+,Eu3+,Dy3+)were designed and synthesized.The structures of the two types of complexes were characterized by a series of methods such as nuclear magnetic resonance spectroscopy,ultraviolet spectrophotometry,infrared spectroscopy and elemental analysis.Fluorescence spectrophotometry was used to construct a novel D-?-A fluorescent probe and a rare earth ion fluorescent probe,then conditions are optimized and methodologies are examined,which were respectively applied to the determination of ibuprofen in vitro.Results The structure of the two types of complexes was confirmed,confirm that the target complex is synthesized.Furthermore,the two types of fluorescent probes were screened for concentration,pH,temperature,time,etc.,and the optimal conditions of the fluorescent probe were obtained.Fluorescent probes are methodologically investigated,for the A1 complex fluorescent probe,the ibuprofen concentration shows a good linear relationship in the range of1.0×10-75.0×10-6mol/L,and the RSD of low,medium and high concentration of ibuprofen both are less than 5.00%.The recovery rates of low,medium and high concentrations of ibuprofen were?96.7±2.91?%,?99.1±1.51?%,and?99.8±1.55?%,respectively.The RSD values of ibuprofen stability within 10 h at room temperature were less than 5.00%.The average sample recovery rate is in the range of 99.8%103.5%,and the relative standard deviation RSD is in the range of 0.63%1.3%.The RSD of ibuprofen was less than 2.00%in vitro content determination;In addition,for the rare earth complex fluorescent probe,the ibuprofen concentration shows a good linear relationship in the range of 8.0×10-82.0×10-6mol/L,and the RSD of low,medium and high concentration of ibuprofen both are less than 5.00%.The recovery rates of low,medium and high concentrations of ibuprofen were?98.3±4.08?%,?101.2±4.71?%,and?99.4±2.34?%,respectively.The RSD values of ibuprofen stability within 10 h at room temperature were less than 5.00%.The average sample recovery rate is in the range of 99.0%105.0%,and the relative standard deviation RSD is in the range of1.5%3.1%.The RSD of ibuprofen was less than 2.00%in vitro content determination.Conclusions In this study,a D-?-A type organic complex containing triphenylamine was synthesized and its structure was confirmed.As a fluorescent probe,it is more convenient and sensitive to determine the content of in vitro ibuprofen.A new method for the determination of ibuprofen is established.The important idea of D-?-A organic complex is used to detect ibuprofen as a fluorescent probe have been provided. |
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