Study On The Chemical Constituents Of Tilia Mongolica Bark And Wood

Tilia Mongolica Maxim, a member of the tiliaceae in the Tilia L. family, is mainly distributed in the areas of inner mongolia. Recently, it was reported that Tilia Mongolica had inhibiting effect on Anoplophora glabripennis. In order to understand deeply and use correctly, know more of its main chemical composition, we carried out the study on chemical constituents of Tilia Mongolica bark and wood.Different kinds of extract in the bark and wood of Tilia Mongolica were obtained by organic solvents respectively. Five compounds were isolated and purified from the extracts by utilizing macroporous resins column chromatophy,silia gel column chromatophy,preparative thin layer chromatophy (TLC),recrystallization and High Performance Liquid Chromatography (HPLC) techniques. The structures of compounds were compared by IR,1D and 2D-NMR (DEPT,HMBC,HSQC,~1H,~1H-COSY) datas and literatures. They are gultinol(1),β-amyrin acetate(2), lupeol(3) and penadecanoic acid(5) respectively. Among them, penadecanoic acid was isolated from the genus for the first time. 1,6-Anhydro-beta-D-glucopyranose and Bis(2-ethylhexyl) phthalate were identified from the genus for the first time by GC-MS. In the meantime, comparisons of chemical compositions were studied by different extraction methods (SD,MAE,UWE,SDE). A review on the triterpenoid compounds obtained from Tilia Mongolica Maxim was given in term of NMR spectra characterics and could be used as reference to identify the structures of triterpenoid by the NMR.