| OBJECTIVE: To investigate the correlation of TKTL1 expression with the clinical pathological features in human gastric cancer.METHODS: The expression of TKTL1 was examined by using immunohistochemistry (SP method) in normal stomach mucosa,paratumoral tissues, gastric cancer tissues and node matastasis.TKTL1 protein expression was also examined in normal stomach mucosa and gastric cancer tissues with western blotting. The relationship between its expression and clinicopathological features was statistically analyzed in gastric cancer.RESULTS:1. The positive rate of TKTL1 expression was 21% in normal gastric mucosa tissues, in paratumoral tissues and gastric cancer tissues was 37.8% and 68.4% respectively. The expression of TKTL1 in cancer tissues was significantly higher than that in the paratumoral tissues and normal stomach mucosa tissues (P<0.001).2.The expression of TKTL1 in gastric cancer tissues was not statistically related with the age, sex, tumor size and the degree of tumor differentiation(P>0.05).3. The positive rate of TKTL1 expression was 35.3% in mucous layer and submucous layer, and the positive rate of TKTL1 expression was 82.5% in muscular layer and serous layer. The two groups had obviously difference(P =0.001). High TKTL1 expression was significantly correlated with depth of tumor invasion (P = 0.024), UICC TNM stage of tumor (P = 0.006) and lymph node metastasis (P =0.003).4. According to the results of western blot, TKTL1 protein of 11/13 from normal gastric mucosa tissues lost but overexpression of TKTL protein was from all samples with gastric cancer tissues.CONCLUSIONS: The expression of TKTL1 was significantly increased with immunohisto- chemistry and western blotting in gastric cancer tissues. High TKTL1 expression was significantly associated with the depth of cancer invasion, tumor UICC TNM stage and lymph node metastasis, but not related with the age, sex, tumor size and the degree of tumor differentiation. |
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