Effect Of High Levels Of Glucose On Expressions Of Vascular Endothelial Growth Factor, Erythropoietin, Erythropoitin Receptor In Mouse Retinal Müller Cells

【Objective】To observe the expressions of vascular endothelial growth factor(VEGF),erytbxopoietin(EPO),erythropoitin receptor(EPOR) in different levels of glucose in the retinal Mtiller cells of mice.So as to investigate the role of M(u|¨)ller cells in the progress of diabetic retinopathy.【Methods】(1) Adopt enzyme digestion to culture the M(u|¨)ller cells of mouse at postnatal 7 day.(2) Divided into 4 groups accordding to the concentrations of glucose in DMEM:25mmol/L,30mmol/L,40mmol/L, 50mmol/L,and the 25mmol/L group was as the control group.Cultured for 1 day,3 days,5 days.(3)The expressions of VEGF mRNA,EPO mRNA,EPOR mRNA were detected by RT-PCR.【Results】The expressions of VEGF mRNA,EPO mRNA,EPOR mRNA in retinal M(u|¨)ller cells were significantly enhanced in the high glucose group which increased obviously(P＜0.05),but the difference between 50mmol/L group and the 40mmol/L group did not have statistic significance(P＞0.05).【Conclusion】(1)With glucose concentration elevating,VEGF expression of cultured retinal M(u|¨)ller cells increased.(2) With glucose concentration elevating,EPO,EPOR expression of cultured retinal M(u|¨)ller cells increased.Therefore,the upregulation of the expression of VEGF,EPO,EPOR in the retinal M(u|¨)ller cells in DM patients may be contribute to diabetic retinopathy.