The Effect Of Ultrashort Wave Therapy On Skeletal Muscle Ischemia-reperfusion Injury Repair In Rats

ObjectivesThrough the detection of IGF-1 in rat skeletal muscle ischemia-reperfusion injury model, expression and observation to understand the ultrashort wave therapy on skeletal muscle ischemia-reperfusion injury in the healing role.MethodsCheck the health of 24 adult SD rats,weighing roughly equal. Using random number table to select 6 as the blank group (O group), 18 the use of the remaining free surgery after femoral artery occlusion producing experimental skeletal muscle ischemia-reperfusion injury model. After the success of the model, according to the former method were randomly divided into I/R, I/R without heat, I/R Micro heat three groups,I/R group without heat, I/R group of micro-heat followed by 10 minutes to give varying degrees of ultrashort wave treatment.The two groups were treated six days.The mice were 8 days after cervical dislocation line method executed operative side skin incision,exposed gastrocnemius observed muscle injury healing. Observation of the operative side after the gastrocnemius check for HE staining and immunohistochemical staining to observe the degree of muscle tumefaction ,muscle fiber with the situation,myogenic cells in the growth of new and the expression of IGF-1 intensity differences.Results1. macroscopic observation: O group was as normal muscle tissue. I/R group with more pronounced tumefaction of muscle tissue can be seen scattered bleeding points, I/R without heat, I/R group of micro-heat muscle tissue tumefaction less and no obvious bleeding point.2.HE staining were observed: O group of muscle fibers were arranged regularly.I/R,I/R without heat,I/R micro-heat group compared with the difference between muscle fibers and I/R group had less myogenic cells in the growth of new and neovascularization than I/R without heat, I/R micro-heat group.3. The integrated optical density(IOD) of IGF-1: I/R withoutheat, I/R group of micro-heat samples IGF-1 expression than the O, I/R group,there is statistical significance (p<0.05). I/R group of micro-heat than in I/R without heat group IGF-1 expression more , there is statistical significance (p<0.05).Conclusions1.Ultrashort wave treatment may increase IGF-1 expression and thepromotion of skeletal muscle I/R injury to heal.2.Ultrashort wave Treatment of skeletal muscle I/R injury in an effective and micro-heat treatment than that without heat treatment better.