| Objectives Retinal ischemia/reperfusion(I/R) injury results in generation of reactive oxygen species (ROS). The aime of this study was to investigate whether delivery of the manganese superoxide dismutase(MnSOD) gene could rescue the retinal ganglion cells (RGCs) damage induced by I/R.Methods I/R injury to the retinas was induced in rats by elevating intraocular pressure for one hour and reperfusion was established immediately afterward. One eye of each Wistar rat was pretreated with recombinant adeno-associated virus containing the the human MnSOD (rAAV-MnSOD) (group A) or only the rAAV (group B) and delivery by subretinal injection 21-day prior to initiation of the procedure. The Contralateral eye served as a control (group C) . The morphological changes of the retinal nerve fibre layer (RNFL) and inner plexiform layer (IPL) were observed and the activities of MnSOD were measured by H&E staining and enzyme linked immunosorbent assay(ELISA) 6h, 24h and 72h after the I/R injury. At 7d ,14d and 28d post injury, RGCs were quantitated by fluorogold retrograde labeling.Result At 6h,24h and 72h after I/R injury, The damages of RNFL and IPLwere more mild than those in group B Jess edematous , vacuolar degeneration and Karyopycnosis was soften. The activities of retinal MnSOD in group A were higher than those in group B and group C (P<0.05) 6h,24h,72h after I/R injury. However, The number of RGCs in group A was higher than those in group B 7d, 14d and 28d after I/R injury (P<0.05).Conclusion rAAV-MnSOD could protect the RGCs from retinal ischemia/reperfusion injury in rat, and is a potential therapy for ischemic retinopathy such as glaucoma, diabetic retinopathy, retinal vascular obstruction and so on.
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