| Objective To explore the role of TLR4 and TNF-αin myocardial ischemia reperfusion injury(MI/RI) when OPCABG by observing the dynamic TLR4 and TNF-αexpression changes at mRNA and relation between them.Materials and methods Wistar rats were randomly divided into Sham and IR group and the rats were killed according to different reperfusion time(0,1,6,12 and 24 hours).TLR4 expressions at mRNA levels were detected by RT-PCR respectively. Myocardial TNF-αwas determined by ELISAResults(1) Compared to Sham group,TLR4 mRNA level was upregulated in myocardium in IR group and peaked at 1 hour of reperfusion.(2) Concentration of TNF-αin IR group was significantly higher than that of Sham group at corresponding time points(all P＜0.05),and myocardial TLR4 mRNA level correlated positively with myocardial TNF-α(r=0.728,P＜0.01).Conclusions Expression of TLR4 might play an important role in MI/RI through promoting myocardial TNF-αexcretion.
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