Expression Of Interleukin-17 In Placenta And Peripheralblood Of Patients With Preeclampsia

Background and Objective At present,the pathogenesis of preeclampsia remains unclear.According to the study has confirmed,it relates to the Th1,Th2,regulatory T (Treg) cells in immune factors.IL-17-secreting Th17 cells are a class of newly discovered T helper cell subsets.It play a role in Pro-inflammatory response, autoimmune diseases,tumour allograft rejection and so on.This research is to investigate the relationship between IL-17,Th17 cell and preeclampsia.Methods Thirty-two patients with severe preeclampsia and thirty normal pregnant women as controls were investigated.The concentration of IL-17,IL-6 and TNF-αin serum was measured by enzyme linked immunosorbent assay(ELISA);The expression of IL-17 mRNA in placental specimens was detected by real-time quantitative polymerase chain reaction(real-time PCR).Results(1).There were no statistical difference between control and preeclampsia group in maternal age(P>0.05).The gestational age and the neonatal weight in preeclampsia group were significantly lower than those in normal group(P<0.05).(2). Comparing the normal group,the level of IL-17 in maternal serum was higher in preeclampsia group(P<0.01).The gestational age didn't affect IL-17 expression (P<0.05).(3).The level of IL-17,IL-6 and TNF-αin maternal serum was higher in preeclampsia group than control(P<0.05,P<0.01 respectively).In preeclampsia group,there are positive correlation between IL-17 and IL-6(r=0.402,P<0.05).(4). Compared with the control group,the expression of IL-17 mRNA was higher in preeclampsia placentas(P<0.05).Conclusions IL-17 may relate to the pathogenesis and pathophysiology of preeclampsia;Th17 cells may be associated with Th1,Th2 and Treg cells and mediate with the occurrence of preeclampsia.IL-17 may be mediated inflammation directly.It may be through the promotion of IL-6,TNF-αproduced indirectly involved in the pathogenesis of preeclampsia.Increasing expression of IL-17mRNA in local maternal-fetal interface may undermine the immune tolerance and lead to preeclampsia or other pregnancy related to immune abnormalities.