| Though more and more evidences point ozone-oxygen to be a great prospect in the pain management field, potential risks of this air mixture should be well investigated owing to its potent oxidative property. Considering the scanty of reports about its impact on neurons, we chose spinal neurons in vitro as our subjects. On the 6-7th day of culture, spinal neurons were induced to endure different concentration (20, 40 and 60μg/mL) of ozone for twenty minutes and then incubated back in the incubator for about 2.5 hours. Ozone was introduced into the culture flask immediately after it was made hypobaric by a vacuum pump. Morphological change was observed under inverted phase-contrast microscopy and endocellular superoxide dismutase (SOD), endocellular malondialdehyde (MDA), lactate dehydrogenase (LDH) were detected to examine the reaction between the oxidant and spinal neuron anti-oxidative system. Protein analysis of Bcl-2, Bax and MAP2 was then performed in an attempt to explore the mechanism of cell death. Our results showed that spinal neurons were vulnerable to ozone toxicity and morphological injury could be observed in the 20μg/mL concentration group and became obvious at higher concentrations. The anti-oxidative system of spinal neuron was not effective enough to cope with the potent oxidative air mixture. SOD elevated in parallel with MDA and LDH. Both necrotic and apoptotic mechanisms were involved in the neuron death process.
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