The Research Of Rh Blood Type Gene Based On The Characteristic Sequence And Chaos Game Representation

The Rh blood type system is one of the most complex of the known human blood type system, the clinical significant of which is only next to the ABO blood type system. It can cause transfusion reaction and hemolytic disease of the feyus and newborn(HDN) because of the pregnancy or blood transfusion of ABO,Rh blood type incompatibility in clinical practice of blood transfusion.The main contents are listed as follows:1. According to the chemical structure classification of a, t, c and g, these concepts ofσ-,τ- andστ-characteristic sequences have been presented and promoted into protein sequence, furthermore, the graph representation is introduced, and protein sequence is simplified to be (0,1) sequence, these characteristic sequences are applied to 18 protein sequences of three different kinds of structure(αhelix,βpucker,αβ), so we obtain some structural characteristic of protein sequences of different structure. From another point of view, according to the relation of the molecular weight and degeneracy of the amino acids, another concept of DNA characteristic sequences(ω-)is presented, and promoted into protein sequence, furthermore, through the graph representation, protein sequence is simplified to be (0,1,2) sequence, we can know that both RHD and RHCE genes all prefer to use the amino acids with small molecular weight and high degeneracy, by comparing the graph representation of the RHD and RHCE characteristic sequences.2. This paper use the Chaos game representation (CGR) of protein sequences based on the detained HP model, it gives the CGR of the protein sequences of RHD gene, which can be regarded as characters map of the protein's secondary structure of RHD gene. This paper still gives the CGR of the DNA sequences of RHD gene, it also compute the corresponding probability matrix for the second-order Markov Chain model. From the probability matrix, we can see the usage preference that the third base of the codons in the DNA sequence of the RHD gene.3. This paper propose another genomic genetic codes: quasi-amino acids coding.The result of the relative usage degree of 78 human genes based on the quasi-amino acids coding indicates that these characteristic is not only consistent with the result of Shi Xiufan et al but more obvious, which show that the quasi-amino acids coding is more rational and scientific.It also compute the preference and the QRFN3'of synonymy codon based on quasi-amino acids coding of RHD gene,we obtain the preference to the synonymy codon of RHD gene and the preference to the base behind the synonymy codon.The innovations are listed as follows: 1. Proposing a new kind of characteristic sequence(ω-).2. Proposing a new kind of genomic genetic codes: quasi-amino acids coding, give out QRSCU and use it to research on the Rh blood type system.