| Depressing the gastrointestinal side effect of aspirin and increasing the stability of Eugenol, Yandureqing was synthesized. In order to offer the experimental basis and rationale on its clinical exploitation and application, the toxicology and pharmacodynamics were researched initially. The paper includes five major contents as follows:1. The established method of detecting AEE in the microemulsion the effective ingredient of Yandureqing microemulsion was detected by method of HPLC. The high performance liquid chromatographic conditions were as followed: ODS column (4.6mm×100mm,5μm) as stationary phase and methanol-water (70:30) as mobile phase; the detection wavelength at 279nm;flowing rate in 1.0ml/min; the calibration curves was a good linearity in the range of 1.25-40μg/ml for AEE at 279nm, the average recovery rate was99.93% with RSD 0.84%. It can be used in quality control.2. Acute-term toxicity test the acute toxicity were studied by Karber method.LD50 of AEE in mice was 10.937g/kg with the 95% confidence interval of 9.309-12.850g/kg; and LD50 of AEE-ME in mice was 5.357g/kg with the 95% confidence interval of 4.388 -6.566g/kg by mouth. AEE was an actually nontoxic drug and AEE-ME belonged to the low toxic preparation.3. Bacteriostasis experiment the bacteriostasis was investigated by cylinder plate method. The MIC of AEE and AEE-ME was 10mg/mL, 5 mg/mL for Bacterium coliO149 separated from swine; 20 mg/mL, 20mg/mL for Staphylococcus aureus; 0mg/mL, 20 mg/mL for aeruginosus Bacillus; 20mg/ml, 5mg/ml for salmonella of diarrhea Alba; 10mg/ml, 5mg/mL for Streptococcus agalactiae. As a result, AEE and AEE-ME had the obvious antibacteriostasis, in which AEE-ME was better than AEE.4. Anti-inflammatory experiment the study was carried out to investigate the anti- inflammatory effect of AEE by using the mouse ear inflammation induced by xylene, the mouse peritoneal capillary permeability increase induced by acetic acid, the mouse paw edema induced by carregenin and the mouse granuloma model induced by cotton ball. The anti-inflammatory mechanism of AEE was observed by assessing the changes of the levels of the PGE2, MDA, and 5-HT in the inflammatory tissues and the NO in blood serum of the mouse paw inflammation model induced by carregenin. These results showed that AEE exhibited remarkable anti-inflammatory activity in xylene-induced mouse ear inflammation, acetic-acid-induced mouse peritoneal capillary permeability increase, carrageenan-induced mouse paw edema and cotton ball induced mouse granulo- ma. AEE reduced the levels of the PGE2, MDA, 5-HT in the inflammatory exudate and the NO in blood serum. The results indicate that AEE has a conspicuous action on anti-inflammation action which primary mechanism is related to the reduction of the levels of the PGE2, MDA, 5-HT in the phlogistic tissues and the NO in blood serum.5. Analgesic experiment The antinociceptive effect of AEE were investigated by using Writhing and Hot plate test, at the same time, the analgesic contribution of AEE were investigated used the drugs which include DDC, Hal, L-Trp and CYP by heat stimulation method;the contents of PGE2 in the whole brain of rats were measured by using UV-spectrophoto metry. It showed that the acetic acid induced writhing and thermal stimulation originated paining in mice were significantly eased by AEE; contents of PGE2 in the whole brain of rats were decreased; the algesia induced by DDC was inhibited; analgesic role induced by Hal was strengthened and stimulated by L-Trp and CYP were degrade. The results showed that the AEE has evid analgesic action; its mechanism has relation to raise the cont of NE, blocke the DA receptor and decreased the contents of PGE2 in the whole brain of rats.
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