The Effect Of EES On The Expression Of C-fos Protein In LPS Hippocampus And The Comparison Of Anti-epileptic Effect Between EES And VPA

The effect of EES on the expression of C-fos protein in LPS hippocampus and the comparison of anti-epileptic effect between EES and VPAObjective: After establishing the chronic LPS kindling model on rats, we could observe the kindling rate and survival rate, as well as the factors that influenced them. In some cells, if the expression of C-fos protein was positive for the immunity reaction, we have also observed how these cells expressed in the areas of CA1, CA2, CA3, DG (Dentate Gyrus, DG) and poDG of rats hippocampus in LPS at any research time, with the method of immunohistochemistry. After the rats have been intervened with different dosage level of EES (ethanol extractive of scorpion) and VPA (valproic acid), we should be engaged in observing the effect of intervention on the positive expression of C-fos protein for immunity reaction, as well as the ethology behavior of model rats. In the end, the contributions of C-fos protein to epilepsy pathogenesy and the potential anti-epileptic effect of EES could be concluded. Method: (1) firstly the 295 adult masculine SD rats should be carefully cleaned, and then 289 of them would be modeled, except 6 rats belonged to the normal control group. (2) if the model was very successful, we could randomize these rats into model control group, VPA intervention group, EES intervention group with different dosage level (such as low dosage level EESL intervention group, moderate dosage level of EESM, and high dosage level of EESH). (3) After the SE (status epileticus) has been provoked, we compared the model group with VPA group, as well as the different dosage level group of EES intervention. The spontaneous seizure of LPS model rats, their seizure severity and times were also observed. (4) With the technology of immunohistochemistry, we have observed the positive expression of C-fos protein in the brains of rats hippocampus which have been classified into above 3 groups at various time. Results: (1) Low dose of Pilocarpine (Pilo) has been repeatedly injected to rats for many times, and the total kindling rate was 93.77 % (271/289), SE seizure rate 85.47 %( 247/289), death rate during the establishment of model 4.85 % (14/289). (2) There were 23 rats died after different intervention in 30days of chronic observation time, and the death rate was 9.62% (23/239), the survival rate 80.62% (216/239) after modeling. (3) Compared to the model group, the frequency and severity of rats SRS (spontaneous recurrent seizure) would be remarkably reduced and relieved after being intervened with VPA, EESM and EESH (P<0.05), so it was with EESL intervention, except for no statistical significance (P>0.05). (4) In model control group, the positive expression of C-fos protein for immunity reaction in the cells of hippocampus, would be predominately increased in 2 days and 7 days respectively. Around 30 days, the expression level got restored and was near to that of normal control group. (5) In the EES moderate and high dosage level group (EESM, EESH intervention group), the cells which had positive expression of C-fos protein for immunity reaction had been predominately reduced, but it had no statistical significance while compared to VPA group (P>0.05). The expression in the group of low dosage level was somewhat reduced, but it had no statistical significance while compared to model group (P>0.05).Conclusion: (1) In this experiment, we have repeatedly injected rats with low dose Pilo for many times, and the rats would be treated with Diazepam as soon as SE (status epilepticus) had been provoked. What we have done could lower the death rate of model rats, and elevate the kindling rate, which would offer us reliable research basis for relevant further study about LPS. (2) the frequency and severity of rats SRS (spontaneous recurrent seizure) would be remarkably reduced and relieved after being intervened with EES of moderate and high dosage level, and it had no statistical significance while compared to VPA group on the anti-epileptic effect (P>0.05), but the result remarkably surpassed the normal control group (P<0.05). (3) the moderate and high dosage level of EES had the function of lowering the expression of C-fos protein for immunity reaction in LPS model rats hippocampus neuron, so we inferred that would be one of the anti-epileptic mechanisms.