Microwave-Assisted Extraction Of Effective Components From Traditional Chinese Medicine And The Seperation Of Tanshinone

Microwave-assisted extraction(MAE) technique has become a fast developing technique in recent years.Compared with conventional methods,microwave-assisted extraction has many advantages.Because of its process simplicity,fast speed,high efficiency,less energy consuming and other advantages,it is used widely in the extraction of effective components from traditional Chinese medicine.In this thesis, MAE was used to extract compound traditional Chinese medicine Ru-Kuai-Xiao(RKX),the main research contents and conclusions are as follows:1,A simple and reproducible method was developed by high performance liquid chromatography to establish the fingerprint of compound traditional Chinese medicine RKX.Under the optimum chromatographic condition,19 main peaks were selected as the common peaks.The relative retention time(RRT),relative peak area(RPA) and similarities were calculated.In addition,both negative control samples and positive control samples were investigated to determine the origins of these common peaks.The results showed that these common peaks in RKX derived mainly from Radix Salviae Miltiorrhizae and Folium Citrus Reticulatae,which testified the TCM theory that these two herbs were the sovereign drugs in RKX and took main responsibility for the pharmaceutical activity.2,27 batches RKX water extract and 23 batches RKX ethanol extract prepared according to conventional technology were tested by the HPLC fingerprint chromatogram method.8 main common peaks in water extract and 3 main common peaks in ethanol extract were selected and signed. The area range of these common peaks was calculated.According to fingerprint chromatogram obtained by conventional technology,the feasibility of using MAE to extract RKX was tested.The results showed that the main common peaks area were within the range when MAE process for water extract and ethanol extract were carrying out under 85℃for 14 minutes with power of 500W and 700℃for 4 minutes with power of 600W,respectively.3,Seven macroporous resins with different polarities and pore sizes were evaluated to determine the static adsorption and desorption of tanshinoneⅡA.Adsorption kinetics curve was obtained and Langmuir equation was used to calculate the constant of adsorption speed.Both Langmuir and Frendlich isotherms were used to describe the interactions between solutes and resins at 25℃.The results showed that HPD-100 displayed higher adsorption and desorption performance for tanshinoneⅡA than other resins.Dynamic adsorption experiments showed that 80% ethanol could desorb tanshinoneⅡA completely,and the desorprion process ended when the desorption solution reached about 10BV.The tanshinoneⅡA content in the final product was increased 2.5-fold from 17%to 42%,with a recovery yield of 80%.