| Objective: At present cleft palate congenital is carried out primarily by cleft palate surgery, but with secondary deformity of the maxilla patients will become even more serious impact whose face, The mainly reason is in which left on the palate after maxillary hard palate of the exposed bone surface caused by scar contracture. To improve the selection of this secondary deformity, cleft palate must be reduced postoperative scar formation in the exposed bone surface. The fibre cell is a mainly effect icatrices formation.This experiment will observe the recombinant human interferonα-2b (rhIFNα-2b) in vitro cleft palate after the source of the exposed bone surface scar fibroblasts biological effects, and explore rhIFNα-2b treatment of cleft palate after the exposed bone surface scar basis.Methods: The exposed skill queen bone is first soft and floury in experiencing, and in observing big ears for the year in a lively way by building a palate, which on white rabbit cracking cicatrices animal model,it will cut scar tissue, the using of in vitro cell culture technology for postoperative cleft palate fibroblasts and primary cultured; to cell factor rhIFNα-2b added to the cultured fibroblasts, respectively, through the MTT method, 3H-TdR infiltration method, flow cytometry methods such as observation, analysis rhIFNα-2b on the palate fibroblasts after the biological behavior of the impacting of data analysis of variance and q test treatment.Results: (1) MTT colorimetric determination of the experimental results showed that: adding rhIFNα-2b after 48h culture, the experimental group compared with the control group, 4000,6000,8000,10000 U / ml concentration group OD value was significantly lower than control group, there is statistical difference on Studing significance (P <0.05, P <0.01). Tipig rhIFN alpha - 2b palatopharyngeal postoperative suppression scarring fibroblast growth proliferation, and presents the inhibition concentration dependence. (2) 3H-TdR incorporation showed: adding rhIFNα-2b after 48h culture, the experimental group compared with the control group, 4000,6000,8000,10000 U / ml concentration group value was significantly lower than control group, the differences were significance (P <0.01). The skill queen cicatrices pointing out that the rhIFNα-2b restraint palate cracks becomes fibre cell DNA combining , and this inhibition showed dose-dependent manner. (3) flow cytometry results showed that: the role of rhIFNα-2b add 48h, the experimental group compared with the control group, 4000,6000,8000,10000 U / ml concentration group in the percentage of G1 phase were significantly higher than that, there is statistical difference Study significance (P <0.05, P <0.01). Cell percentages increase by with the fact that medicine thickness enlarging , DNA composing the earlier stage (G1) cells, DNA synthesis of early (G1) cells, the percentage increasing, showing a dose-dependent manner. Prompted rhIFNα-2b inhibited scar formation after cleft palate fibroblast growth proliferation.Conclusions: rhIFNα-2b on the palate fibroblasts after growing trend and significant inhibition of proliferative activity, suggesting that rhIFNα-2b are cleft palate fibroblasts by negative regulatory factors, which rhIFNα-2b in the prevention and treatment of cleft palate after the exposed bone surface in the formation of scar must have application value.
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