The Relevance Of Brain Tumor Stem Cells And Tumor Pathological Characteristics And Study Of Proliferation And Differentiation Of Brain Tumor Stem Cells

Objective (1) To explore the expression of CD133 and its distribution on paraffin sections from different pathological grade glioma. (2) To explore isolation, proliferation and differentiaton of culturing brain tumor stem cells(BTSCs) from distinct grade gliomas in vitro.Methods The method employed:(1) By immunofluorescence staining and immunohistochemical staining that using CD133 and Ki67 to label on paraffin sections, to observe and compare the distribution of BSTCs from distinct grade gliomas(â… -â…£) with 78 cases of paraffin section. (2) During the process of isolation, culture and identification in serum-free culture medium,and the process of proliferation and differentiation in serum culture medium in vitro, BTSCs from distinct grade gliomas were observed and compared.Results (1) By repairing antigens of brain tumor stem cells, In low-grade gliomas,The expression of CD133 is very low to the existence of scattered and non-clustering phenomenon. In gradeâ…¢,â…£gliomas biopsy, the clustering phenomenon of the CD133+ cells can be observed, and particularly evident in medulloblastoma. In gradeâ…¡-â…£gliomas, the most of CD133+ cells is around the blood vessels or adjacent to blood vessels. The nest-like distribution of CD133 cells are near to the edge of blood vessels. (2) These phenomenon were observed that BTSCs from gradeâ… gliomas could not be cultured to spheroplast, these from gradeâ…¡gliomas can develop to medium -sized spheroplast and these from gradeâ…¢gliomas or gradeâ…£gliomas can develop to larger spheroplast in serum-free culture medium.As against that of BTSCs from gradeâ…¢gliomas or gradeâ…£gliomas, proliferation and differentiation of BTSCs from gradeâ… gliomas is slowly in the early stage.Proliferation and differentiation of BTSCs from gradeâ…¡gliomas is between the above two.Proliferation and differentiation of culturing distinct grade BTSCs were compared with cells number among serum culture groups and serum-free culture groups(P<0.01).Conclusion (1)The number of Brain tumor stem cells is positively related to pathological level of Glioma in paraffin section each other. From low-grade gliomas to high-grade gliomas, the distribution of the brain tumor stem cells is scattered,gathering,and nest-like in the perivascular. (2) BTSCs from distinct grade gliomas is biologically multifarious.BTSCs from gradeâ…¢gliomas or gradeâ…£gliomas is easily obstained. Proliferation and differentiation of these BTSCs is faster than those from the other grade gliomas. Because proliferation and differentiation of BTSCs from gradeâ… gliomas is slowly in the early stage,it is a better experimental subject in the self-renew, proliferation and differentiation of BTSCs.