| Objective:Restoring medical science is the hot spot studying in recent years,whose significance Yue Lai Yue is talked to come people's atten- tion,sort,common restoring process being that the clinical various wound and disease cure process hit the target returns to revolution with the development of advancement of society and medical science.Ideal renova- tion final ought to be that anatomy structure and the physiology function are reached by wound organization recover from the illness completely.That the in the process of actual renovation but,a lot of organization inside and outside factor affects the secondary cell,molecule or disturbance as well as the gene is horizontal to restore process,makes the renovation restoring process being difficult in terms of person's own wish,to develop but appear be out of control thereby.Within shaping the field restoring surgery,type skin is one of the renovation way applying most often at will piece.Since but skin does not have the famous blood vessel in the piece,blood confesses to depend on the subcutaneous blood capillary net only,design that go ahead piece therefore in skin,often must abide by a principle,be skin for piece of the limit comparing of strong point,width growing:Width=2:1,transports rich area,but amounts to3:1in some part blood.The exceeding this range,skin piece of far end pole are easy to incomplete blood necrosis happ- ened.Skin has 10% one 20%'s miss ratio at will piece of transplant- ting,should must adopt this piece of time extra-long skin,sometimes must perform the once "defers" operation,blood in piece provides system,ensures that blood transports in order to rebuilding skin. How to be able to realize this extra-long skin once of piece changing success,is a problem being worth it studying.The experiment studies aim at investigation and discussion surpasses free rein skin injecting the blood vessel Neipi growth factor(VEGF)piece of part combining Oxygen applying hyperbaric oxygen(Hyperbaric,HBO) may improve skin improves piece of survival percentage skin piece of microcirculation,the piece of blood luck increasing skin thereby.Material and method:(1).Divided into group:All the year the rat 12 is 2 group,A group mark according to random assignment principle:6 gods of the earth,blank space contrast the group,the B group: VEGF 6 forms.(2).Combining with HBO,skin piece of preparation:Skin fixes whose long width proportion at will piece in rat abdomen preparation,for3:1(3X1 cm).The barbital sodium(10mg/kg)intravenous with 5% anaesthetizes the queen,molt,prepare skin,the queen who disinfects,piece of far end begins from prefabricating skin,the blood vessel net separates skin under flesh rete and dermis between the tier preparation becomes free rein type skin piece.(3).The skill makes the PU value admeasurement late than 4th day,10th day,12nd day respectively to close among piece of keeping away from, forming skin,Duan.And 12nd day puts a rat to death,is sure to fetch piece of specimen skin,the PFA(poly formaldehyde)solution fixes,makes the wax piece,continuous section immediately with 4%,make routine dyeing observing the detecting organizing form change,the immune group spending law(SP law)analyses the proliferation cell nucleus antigen (PCNA) and micromodule density (MVD).Use SPSS13.0 to build a data base and carry out method difference analysis on result.Result:(1).PU value determines skin there being no statistics significance piece of the difference forming the first two set value.Two set of skin pour different period forming the day afer tomorrow piece in skin piece of equal behaviour for distant Duan bloodstream than the middle piece and close Duan are bad, among them close Duan blood is for two the most enough skin of group's forming the queen piece and a period of time expects there being no statistics significance,not but being hit by with the location value difference in close Duan,distant Duan value have notable difference.(2).HE dyeing: B survival skin of group piece of hypoderm edema having certain degree,the cuticle hyperplasia enlivens,the granulation organization hyperplasia,cell hyperplasia having large area under the cuticle are obvious,the large amount of freshman blood capillary takes form. Piece of hypoderm edema A survival skin of group is had to spread the bleeding under skin obviously,the newborn blood vessel and granulation organization distribute range being smaller than the B group obviously.Lose far end skin piece of complete necrosis , the cell form.(3).PCNA checks:The B survival skin of group piece of middle cell hyperplasia enlivens(PI= 63%),large amount of yellow dyeing of palm fibre proliferation cell nucleus,base the bottom cell hyperplasia is especially obvious,there are large amount of freshman blood capillary in the hyperplasia granulation organization,the A group expresses weaker(PI= 20%), the masculine gender cell falls apart in distribution,the newborn blood vessel is short.Two set of skin have the notable difference piece.(4).skin checks piece of micromodule density (MVD):B the blood vessel bast cell by yellow dyeing of palm fibre having a great quantity set,the end face blood vessel distributes concentrated 45 MVD=cm2,A group blood vessel distribution is less,the yellow dyeing of palm fibre blood vessel bast cell is sparse20MVD=cm2. Two set of MVD have notable difference(P <0.001).Conclusion:The skill queen part injection blood vessel Neipi growth factor(VEGF)combines 1,Oxygen applying hyperbaric oxygen(Hyperbaric, the skin HBO)being cured but reducing the rat abdomen free rein obviously boosts skin surviving piece piece of necrosis rate.2,HBO can improve skin blood vessel density in piece of organization3,the part injection blood vessel Neipi growth factor(VEGF)combines to apply hyperbaric oxygen boosting free rein skin taking form piece of tiny blood vessel.
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