| Objective: To investigate the effects of Celastrol on the proliferation and apoptosis of gastric cancer cells (SGC7901) in vitro, and to evaluate whether Celastrol can be used as synergetic agents in chemotherapy.Methods: The gastric cancer cell line SGC7901 cells were incubated in vitro with 5-FU (5μg/ml) and Celastrol of different concentrations in combination or respectively for 24h,48h,72h. The effects of the treatments on the growth and survival of SGC7901 were determined by MTT assay. Apoptosis was measured using flow cytometry and Expression of NF-κB mRNA was determined by RT-PCR. The data was analyzed by DAS and SAS software.Results:1.The inhibition rates of gastric cancer cells by treatment with Celastrol (20μg/ml, 10μg/ml, 5μg/ml, 2.5μg/ml, 1.25μg/ml) for 24h were 56.46%±1.18%, 48.98%±2.04%, 27.89%±2.36%, 12.93%±1.18%, 3.40%±2.36%; for 48h were 88.50%±2.52%, 79.00%±2.58%, 74.00%±1.63%, 57.00%±1.15%, 42.50%±1.91%; for 72h were 93.06%±1.20%, 80.56%±3.18%, 53.47%±4.34%, 32.64%±5.24%, 22.22%±1.20%. (P<0.05).2. when chemotherapeutic drug (5-FU 5μg/ml) was combined with Celastrol, the inhibition rates for 24h were 70.92%±1.95%, 60.71%±3.06%, 53.06%±2.89%, 44.39%±3.49%, 33.16%±1.95%; for 48h were 88.50%±2.52%, 79.00%±2.58%, 74.00%±1.63%, 57.00%±1.15%, 42.50%±1.91%; for 72h were 95.83%±1.70%, 85.98%±3.40%, 83.33%±1.70%, 71.35%±1.99%, 56.25%±3.80%, which showed a synergetic effect (P<0.05).3. RT-PCR revealed that Celastrol respectively or combined with 5-FU could decrease the expression of NF-κB mRNA markedly; the inhibition was more obviously when combined with 5-FU. Conclusion:1. Celastrol had an ability to suppress gastric cancer cells in vitro,and the synergetic effect became evident when it was combined with chemotherapeutic drug 5-FU.2. Clestrol could induce the apoptosis of SGC7901 cells respectively or in combination with chemotherapeutic drug 5-FU.3. Celastrol or combined with 5-FU could decrease the expression of NF-κB mRNA obviously.
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