To Investigate The Polymorphism Of Matrix Metalloproteinase-9 And Platelet Glycoprotein Ⅲa With Cerebral Infarction.

Cerebral infarction (CI) refers to a disease of ischemia that causes the brain tissue necrosis ,soften and the patients often appear corresponding nervous system symptoms such as hemiplegia and aphasia. The person who suffered from the disease is usually more than 50 years old with coronary arteriosclerosis. It has become a great danger to human health with it's high morbidity and mortality as a kind of multifactor disease that include environmental and inherent factors .In recent years, it has become a new field that researching the potential influence of gene polymorphism and gene mutation to cerebral infarction. The results of the study have illustrated that gene structure and expression abnormal may be the basic reason that causes cerebral infarction, including mutation, shifting, inserting and lacking of the gene.Objective: To study the relevance between the MMP-9 (Matrix metalloproteinase-9) ,GP( Glycoprotein ) IIIa with the disease of cerebral infarction. The level of plasma fibrinogen (FIB), homocysteine(Hcy), lipid such as total cholesterol (TC), triglyceride(TG), low density lipoprotein(LDL), high density lipoprotein (HDL), lipoprotein a(LPa), apolipoprotein A(APOA) and apolipoprotein B(APOB) has been examined.Methods: Fifty-seven patients of cerebral infarction, including 39 male, 18 female are from the Second Hospital of ShanXi Medicine University. The age is from 33 to 82, mean age is 67.86±10.20. Eighty-four healthy person, selected from physical examination,are control group including 52 male, 32 female.The age is from 40 to 74 and the mean age is 62.55±9.22. The level of FIB ,Hcy, lipid ( TC, TG, LDL, HDL, LPa) and apolipoprotein (APOA, APOB) were measured by full-automatic biochemical apparatus. Draw blood cell DNA with potassium iodide(KI) method and distinguish the genotype according to the strip. Compared with the above-mentioned control. Results: The level of plasma TC, TG, LDL, Lpa, APOA , APOB,FIB and Hcy is higher in patients with CI. TC, TG and LDL have no significant difference(p>0.05), while FIB,Hcy are remarkable higher than the control group(p<0.05). Using multifactor analyzing, the plasma level of FIB enters the regression equation. The gene mutation at MMP-9 C-1562T position has remarkable difference, while the mutation at GPIIIaT1565C position is very low in both CI and control group.Conclusion: The relationship between plasma lipid and apolipoprotein with cerebral infarction is not clear and their function is still to be proved in future.Plasma FIB and Hcy are the dangerous factors for the patients with CI. MMP-9 C1562 T gene polymorphism may cause the brain to express high level of MMP-9 and induce brain hurted. The relationship between the polymorphism of GPIIIa T1565C with CI is still to be researched.