Ri Silencing By Small Interfering RNA Promotes Metastasis And Invasion Of Bladder Cancer Cell

Objective To investigate the effect of RI expression silencing caused by vector-mediated RNAi on the migration and invasion of BIU-87 human bladder cancer cell.Methods Construct two specific siRNA expression vector and one no homology control vector , and identify the vectors by restriction endonuclease digestion and DNA sequencing. Transfect the vectors into cell. Detect the efficiency of RNAi through RT-PCR, western blot and immunocytochemistry assay. Observe the morphous change using, and determine the adhesion, migration and invasion ability modify by cell adhesion test, wound healing assay and transwell invasion assay. Finally, detect the modification of matrix metalloproteinases expression in BIU-87 cells and observe the actin cytoskeleton changing by laser scanning confocal fluorescence microscope.Results Enzyme digestion and DNA sequencing verified the vectors constructed correctly. It is exhibited that RI expression was obviously inhibited in the two specific siRNA treatment groups, but the control groups remained the same level. And the cells in two treatment groups becamed overlapped severely, decreased the cell adhesion ability, increased the cell migration and metastasis potential, significantly promoted matrix metalloproteinases expression and reorganized cytoskeleton in which microfilament assemble and extended several lamellipodia.Conclusion Inhibiting RI expression will induce a series of behavior modify of BIU-87, and increase cell migration and metastasis potentia significantly. And it has indicated up-regulation could inhibitor tumor metastasis previously. In conclusion RI could be a valid anti-metastasis and anti-invasion therapeutic target.