Ultrasonography Monitors Treatment Of VX2 Liver Tumor With Steep Pulse

the steep pulse therapy has been introduced, which can kill tumor cells in vivo and vitro. The aim of this study was to compare the ablated area in ultrasonograhy and with that confirmed with histological examinations.PART 1 THE AIM OF THIS PART IS TO OBSERVE THE CONSISTENCY OF THE SP-ABLATED AREA IN ULTRSOUND AND IN PATHOLOGICAL EXAMINATIONSObjective To investigate the consistency of the SP-ablated area in ultrasonography and that in pathological examinations.Material and methods Eighteen rabbits were divided into 6 groups randomly with a animals in each group (0,1,4,7,14,21d).Rabbits were exposed to steep pulse electric field, and then received pathological examinations.Results After the ablation, seven hyperechoic dots representing the needles tracks can be observed in ultrasound images. Then the next five detects ,we can see the hyperechoic dots of the needles, the hypoechoic appearance of the expected area. The histological changes were consistent with ultrasonic images. Pyknosis, karyoclasis and karyolysis were detected in cells exposed to SP.Conclusion the SP-ablated area in ultrasonography were consistent with that in pathological examinations.PART 2 STEEP PULSE THERAPY OF RABBIT VX2 LIVER CANCER MONITORED WITH REAL-TIME ULTRASONOGRAPHYObjective To investigate the effect of ultrasound in plot and real-time monitor with steep pulse ,and pathological examinations laterMaterial and methods thirty-six rabbits bearing VX2 liver cancer were divided into five experimental groups and the coutrol group, which were subjected to steep pulse treatment, respectively. The parameter was 900V/10μs /20Hz/10min. ultrasonography was used to monitor treatment. Pathological examinationsa were performed.Results the hypoechoic image of VX2 liver cancer could be detected before the treatment. the hyperechoic dots representing the needles tracks could be detected when inserted the electric needles, the pathological examinations After treatment, pyknosis, karyoclasis and karyolysis of tumor cells were observed under light mircroscopeConclusion ultrasound can be used to monitor SP therapy in real time.