Elevation Of Plasma Membrane Permeability On Laser Irradiation Of Extracellular Microbubble

Objectives To investigate the feasibility of using laser to blast liposome microbubble contrast medium and the influence to retinoblastoma cell proliferation at the first time, then we discussed the elevation of plasma membrane permeability of retinoblastoma cell on laser irradiation of extracellular microbubble.Methods 1. Laser light with different wavelength were depolied to blast the microbubble by ultimate capacity, then DFY-Ⅱultrasound quantitation analyses diagnostic apparatus was used to detect the blasting rate of the microbubble, and we could get which kind of laser light could blast the microbubble successfully through comparing the blasting rate. 2. The Nd:YAG laser which could blast the microbubble successfully was used to blast the microbubble with different parameters, and with which parameter could blast microbubble most effectively was decided. 3. The survival ability of the retinoblastoma cell were detected after Nd:YAG laser or laser-microbubble combination system irradiation by MTT. 4. The membrane permeability was evaluated by flow cytometric assaying using propidium iodide (PI) and fluorescein diacetate (FDA) after Nd:YAG laser or laser-microbubble irradiation, and contrasted with the ultrasound.Resluts 1. When the total energy≥250mJ, Nd:YAG laser could blast the liposome microbubble successfully; higher energy resulted in higher blasting rate; when the total energy≥500mJ, the blasting rate >90%. 2. When the total energy of the Nd:YAG laser≥500mJ, the survival ability of the retinoblastoma cell were decreased, and if the cells were irradiated with the laser-microbubble combination system, the survival rate was lower than that with the laser merely. 3. The membrane permeability was improved both in the laser-microbubble combination system and laser merely, and the former has higher membrane permeability, but lower than the ultrasound -microbubble system.Conclusion 1. Both the Ar-green laser and Novus Varia poly-wavelength laser couldn't blast the liposome microbubble successfully. 2. Nd:YAG laser could blast the liposome microbubble successfully; higher energy resulted in higher blasting rate. 3. When the total energy of the Nd:YAG laser was high enough, the survival ability of the retinoblastoma cell was decreased, and if the cells were irradiated with the laser-microbubble combination system, the survival rate was lower than that with the laser merely. 4. The membrane permeability of the cells could be improved both by the laser and laser-microbubble combination system.