Effect Of Butylphthalide On The Expression Of Glial Fibrillary Acidic Protein And Vascular Endothelial Growth Factor Of The Alzheimer Disease Model Rats

Objectives To observe the expression of glial fibrillary acidic protein (GFAP) and vascular endothelial growth factor (VEGF) in the CA1 region of hippocampi of Alzheimer disease (AD) model rats, and to determine the effect of butylphthalide on them. To explore the role of blood-vascular factors in Alzheimer disease's invasion and the potential mechanism of butylphthalide on AD.Methods Sixty healthy male Sprague-Dawley (SD) rats were selected and then divided into three groups randomly, which were the AD group, the butylphthalide group and the control group. The AD model was set up by injection with beta-amyloid peptide 1-42 (Aβ_1-42) into the hippocampus stereotacticly. Learning and memory abilities were tested through Y-type electric maze before and sixty days after operation. The morphology of neurons in the CA1 region of hippocampus was determined by HE staining sixty days after operation. The deposition of A6 was measured by immunohistochemistry(IH). Both of the expression of GFAP and VEGF were observed by IH.Results1. the learning and memory abilities of ratsComparison among groups showed no significance (F=0.041, P=0.960) before operation. Sixty days after operation, significance was showed among groups (F=11.682, P<0.001) . Comparison between the AD group and the control group showed significance (P<0.001) . Comparison between the butylphthalide group and the AD group showed significance (P<0.001) . Comparison between before and sixty days after operation of the AD group and the butylphthalide group showed significance (P<0.001) , which of the control group showed no significance (P=0.309>0.05) .2. HE stainingMorphology: In the AD group, normal neurons in the CA1 region of hippocampus decreased greatly, the neurons had irregular arrangement, the edge of many cells was unclear, and the nucleus and nucleolus of many neurons became ambiguous. The butylphthalide group showed some neurons necrosed, the neurons had almost regular arrangement, and there were more normal neurons than the AD group. In the control group, the neurons showed regular arrangement, with distinct edges and clear and obvious nucleus and nucleolus, and no significant necroses of pyramidal neurons was found.Density of the neurons in the CA1 region of hippocampus: There was significant difference among the three groups (F=113.27, P<0.001). Compared with the control group, the AD group showed significant difference (P<0.001) . Compared with the AD group, the butylphthalide group showed significance (P<0.001) .3. Expression of 6E10.There was buffy deposition in the hippocampus of the AD group and the butylphthalide group, and no buffy deposition in the hippocampus of the control group.4. Expression of GFAPThere was significant difference among groups (F=65.35, P<0.001). Compared with the control group, the AD group showed significant difference (P<0.001) . Compared with the AD group, the butylphthalide group showed significance (P<0.001) .5. Expression of VEGFThere was significant difference among groups (F=46.82, P<0.001). Compared with the control group, the AD group showed significant difference (P<0.001) . Compared with the AD group, the butylphthalide group showed significance (P<0.001) .Conclusions1. The butylphthalide can improve the learning and memory abilities of the AD model rats obviously.2. The butylphthalide's protection on AD may be contected with inhibiting the expression of GFAP, increasing the expression of VEGF, defending the neurovascular unit and raising the cerebrovascular reserve.