| Objective To examine the possible difference in genomic DNA between nasopharyngeal carcinoma drug-resistant cells(CNE-1/Taxol, HNE-2/Taxol,5-8F/Taxol) and the parent cells.Method 1.The colony formation assay was repeatly carried out to detect the resistance index of paclitaxel-resistance nasopharyngeal carcinoma cells.2.Nasopharyngeal carcinoma drug-resistant cells and the parent cells were detected by comparative genomic hybridization in the gains and losses of chromosomes.Results 1.The half inhibiting concentration of drug-resistant cells (CNE-1/Taxol,HNE-2/Taxol,5-8F/Taxol) are 9.61±0.43,11.48±0.52,9.96±0.48 ng/ml and the half inhibiting concentration of parent cell lines are 1.14±0.05,1.78±0.08,1.63±0.06ng/ml.So the resistance index of paclitaxel-resistance nasopharyngeal carcinoma cells respectively are 8.43±0.42,6.45±0.31,6.11±0.35.2.As for the parent cell lines,the common gains are 3q21-qter,5p13-pter, 20q11-qter and chromosome12.The common losses are chromosome 18,X.3.As for the nasopharyngeal carcinoma drug-resistant cells,The common gains are 3q21-qter,5p13-pter,8q21-qter,chromosome12 and 20q11-qter.The common losses are 10q11-qter,chromosome 18,X.4.Copared with the parent cells,the specicial common gain of drug-resistant ceils is 8q21-qter.Conclution:1.There are characteristic chromosome changes in nasopharyngeal carcinoma drug-resistant cells and the parent cells.Both sameness and difference exist in the chromosome changes of NPC cell lines and drug-resistant cell lines.2.The common gains of three parent cell lines are 3q21-qter,5p13-pter, 20q11-qter and chromosome12.The common losses are chromosome 18,X.It is possible to find new genes which cause nasopharyngeal carcinoma in these regions.3.Compared with the parent cells,the specicial common gain of drug-resistant cells is 8q21-qter.Further studing on the region,it is possible to give new clue on nasopharyngeal carcinoma drug-resistant mechanism.
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