Study On Synthesis And Biological Activity Of Genistein Acetylferulic Acid Esters

This paper consists of four parts.Chapter 1: In this section, the status of flavoniods and ferulic acid and its derivative were reviewed. The significance and direction of the research were elucidated.Chapter2: Two new compounds: genistein-7-acetylferulic acid ester and genistein-7,4'-di-acetyl-ferulic acid ester were firstly synthesized from ferulic acid and genistein based on Schotten-Bamann reaction. The structural characterization of the target compounds were conducted by, IR, ¹HNMR, ¹³C NMR and MS spectra.Chapter 3: The abilities of free radical scavenging and ferrous iron chelating and inhibition of hydroxyl radicals-mediated DNA degradation of genistein acetylferulic acid esters were evaluated by using various chemical and biological assays. The results showed that the abilities of two genistein acetylferulic acid esters were stronger than genistein on scavenging free radical of·DPPH and·OH. Two esters exhibited almost the same good protecting effect on DNA damage caused by hydroxyl radical as genistein. Ferrous ion chelating activities of two esters appeared to have abated somewhat than genistein. Furthermore, We studied their structure-effective relationship in this part.Chapter 4: The interactions of genistein acetylferulic acid erters with calf thymusDNA(ctDNA) were investigated in this section by using means of UV spectra, fluorescent spectra and viscosity, respectively. The results showed that the UV spectra of genistein acetylferulic acid erters showed obviously hypochromism under the presence of ctDNA. In addition, the tested compounds could make the fluorescence intensity of EB-DNA system decreased sharply, and also increased the relative viscosity of ctDNA aqueous. The experimental results suggested that genistein acetylferulic acid erters could mainly bind to ctDNA by intercalation.