Effect Of Chlorogenic Acid, Caffeic Acid, And Ferulic Acid On Inhibition Of The Inflammatory Response Of HMECs Induced By Activated Complement Alternative Pathway

Object: To investigate the effect of chlorogenic acid, caffeic acid, and ferulic acid on expression of adhesion molecules during inflammation response induced by activated alternative pathway on human microvascular endothelial cells(HMECs) with the use of cobra venom factor(CVF).Methods: CVF was used to activated serum complement pathway, after exposure of HMEC to activated complement for various times, supernatant was removed and assayed for expression of ICAM-1, IL-6, IL-8, t-PA, PAI-1 by using reagent kits.HMECs were exposured to various concentrations of CGA, CA, and FA, respectively,for 24 h, then the cell viability was determined by MTT method, the appropriate concentration was used to the test. No effect of CGA, CA, and FA with different concentrations were used to detect their concentration on ICAM-1, IL-6, IL-8, t-PA,PAI-1. And the affecting concentration on cell viability was used to further study on endothelial cell damage.Results: The activation of the alternative pathway of complement product was acted on HMEC,leading to HMEC activation. Up-regulating the expression of ICAM-1,IL-6, IL-8, t-PA, PAI-1 and increase the oxidation levels of endothelial cells. 0.1, 0.5mmol·L-1 of CGA, CA, FA on cells without injury, and 1.0, 3.0, 5.0 mmol·L-1 of CGA,CA, FA on cells significant injury, cell viability decreased significantly. 50, 100, 250?mol?L-1 of CGA, CA, and FA can decrease the content of ICAM-1, IL-6, and IL-8.Treated with more than 1.0 mmol·L-1 of CGA, CA, and FA for 24 h, HMECs obviously deformed and shrinked. The release of LDH was significantly increased at24 h time point. Apoptosis significantly upregulated after treated by CGA, CA, and FA compared with normal group.Conclusion: 50, 100, 250 ?mol·L-1 of CGA, CA, and FA can inhibit the expression of ICAM-1, IL-6, IL-8 caused by the activation of the alternative complement pathway.More than 1.0 mmol·L-1 of CGA, CA, and FA can cause endothelial cell damage,causing apoptosis of HMECs.